STBCi320-A

SFC031-03-03

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name STBCi320-A
Alternative name(s)
SFC031-03-03
Cell line type Human induced pluripotent stem cell (hiPSC)
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obsolete_Parkinson's disease
SUSMi005-A-2
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obsolete_Parkinson's disease
SUSMi005-A-3
(SNCA3X 2KO C1, SNCA3X 2KO C2)
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obsolete_Parkinson's disease
SUSMi005-A-4
(SNCA3X 3KO C1, SNCA3X 3KO C2)
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obsolete_Parkinson's disease
SUSMi005-A-5
(SNCA3X 4KO C1, SNCA3X 4KO C2)
Donor diseases:
obsolete_Parkinson's disease

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg STBCi320-A
BioSamples SAMEA5859484
ECACC 66541211
Cellosaurus CVCL_WU51
CLO CLO_0103005
Wikidata Q98132854

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson disease (primary disease)
Sporadic Parkinson's disease / dyskinesia
The donor is affected.

External Databases (Donor)

BioSamples SAMEA5859483

hIPSC Derivation#

General

Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Age of donor (at collection) 55-59
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection Cytotune 2.0
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
Morphology pictures

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Identity to parent fibroblasts confirmed. Normal karyotype..
Passage number: 10
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)