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STBCi023-B

SFC829-03-04

iPSC line

Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: confluence
Magnification: 4x
Timepoint: confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
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General#

Cell Line

hPSCreg name STBCi023-B
Alternative name(s)
SFC829-03-04
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
STBCi023-A
(SFC829-03-02)
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SNCA
Donor diseases:
Parkinson disease
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STBCi019-C
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SNCA
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Parkinson disease
EDi001-A-1
(AST22-C, AST23-C)
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SNCA, SNCA
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Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
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SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
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SNCA, SNCA, SNCA, SNCA
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Parkinson disease
STBCi083-A
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SNCA
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Parkinson disease
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SNCA
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(SFC831-03-03)
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Parkinson disease
EDi008-B
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SNCA, SNCA, SNCA, SNCA
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(AST22, AST23, SAMEA3319992)
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SNCA, SNCA, SNCA
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(SFC834-03-10)
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STBCi025-B
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GBA1
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Parkinson disease
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(SFC029-03-04)
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STBCi040-B
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(SFC081-03-02)
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STBCi042-A
(SFC848-03-02)
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GBA1
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Parkinson disease
STBCi042-B
(SFC848-03-04)
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GBA1
Donor diseases:
Parkinson disease
STBCi042-C
(SFC848-03-06)
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GBA1
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STBCi043-A
(SFC120-03-03)
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STBCi043-B
(SFC120-03-04)
Donor diseases:
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STBCi040-C
(SFC029-03-01)
Donor diseases:
Parkinson disease

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi023-B
BioSamples SAMEA104493650
Cellosaurus CVCL_RB77
Wikidata Q54956397

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
SNCA (target)
4q22.1
SNCA A53T

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493649

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 45-49

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Latest released batch

Culture medium mTeSR-1
Passage method EDTA
Surface coating Matrigel
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)