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EDi008-B

G51D-4, EDINi008-B, EDIi008-B, SAMEA3174606

iPSC line

In Stock
At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: x4
Timepoint: Confluence
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name EDi008-B
Alternative name(s)
G51D-4, EDINi008-B, EDIi008-B, SAMEA3174606
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider
Depositor University of Edinburgh (ED)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg EDi008-B
BioSamples SAMEA3174606
Cellosaurus CVCL_LE56
ECACC 66540061
CLO CLO_0037342
Wikidata Q54831978

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information
Sex female

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Parkinson disease
Parkinson disease in the OLS
The donor is a carrier of a disease-associated mutation and affected.
Genetic variants
SNCA (target)
4q21
NM_000345.3:c.152G>A
NP_000336.1:p.Gly51Asp
SCV000115329.3
Donor known to carry SNCA: G51D mutation.

Karyotyping (Donor)
Has the donor karyotype been analysed?
No

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor
All cell lines of this donor's relatives
Has mother:

External Databases (Donor)
BioSamples SAMEA3174344

hIPSC Derivation#

General
Source cell type
fibroblast
fibroblast in the OLS
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Reprogramming method
Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
Yes
Methods used
PCR

Vector free reprogramming
Type of used vector free reprogramming factor(s)
None

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch
Culture medium Essential E8
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XX,der(15)t(1;15)(q12;p11.2)[9]/46,XX[2]
Passage number: 11
Karyotyping method: G-Banding

Other Genotyping (Cell Line)