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STBCi295-B

SFC847-03-08

iPSC line

Stock unknown
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name STBCi295-B
Alternative name(s)
SFC847-03-08
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg STBCi295-B
BioSamples SAMEA5859486
ECACC 66541210
Cellosaurus CVCL_WU53
CLO CLO_0103003
Wikidata Q98132853

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 75-79

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson disease (primary disease)
Genetic variants

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104620717

hIPSC Derivation#

General

Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Age of donor (at collection) 75-79
Passage number reprogrammed 4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Morphology pictures

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Karyotype abnormalities: none detected
Passage number: 11
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)