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SIGi001-A-18

SIGi001-A-12 Bi-Allelic MAPT HA-tag #U28-12-P29-5

Gene-edited iPSC line

Not-for-profit fee: £1700 per vial
Available within next 12 months
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name SIGi001-A-18
Alternative name(s)
SIGi001-A-12 Bi-Allelic MAPT HA-tag #U28-12-P29-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
SIGi001-A-1
(iPSC0028 SLC17A7/GFP E3)
SIGi001-A-2
(iPSC0028 SLC17A7/GFP C3)
SIGi001-A-3
(iPSC0028 MAPT P301L C4)
SIGi001-A-4
(iPSC0028 MAPT P301L D4)
SIGi001-A-5
(iPSC0028 MAPT P301S 1C9C4)
SIGi001-A-6
(iPSC0028 MAPT P301S 1B9C9)
SIGi001-A-7
(iPSC0028 MAPT P301S 2G2B7)
SIGi001-A-8
(iPSC0028 MAPT P301S+Ex10+16/Clone 7D11A7)
SIGi001-A-9
(iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1)
SIGi001-A-10
(iPSC0028 MAPT P301S+Ex10+16/Clone 7G4A8)
SIGi001-A-11
(iPSC0028 MAPT P301S+Ex10+16/Clone 7C6A4, SAMEA4451118)
SIGi001-A-12
(iPSC0028 - BiAllelic MAPT_Ex10+16T/Clone 1F5-D12, SAMEA104237570)
SIGi001-A-13
(iPSC0028 – MonoAllelic MAPT_Ex10+16T/Clone 1D01-11)
SIGi001-A-14
(SIGi001-A Bi-Allelic MAPT HA-tag)
SIGi001-A-15
(SIGi001-A Dox inducible NGN2)
SIGi001-A-16
(SIGi001-A-9 Bi-Allelic MAPT HA-tag)
SIGi001-A-17
(SIGi001-A-9 Dox inducible NGN2)
SIGi001-A-19
(SIGi001-A-12 Dox inducible NGN2)

Provider

Depositor Bioneer (BION)
Owner Bioneer (BION)
Distributors
EBiSC
Derivation country Denmark

External Databases

hPSCreg SIGi001-A-18
BioSamples SAMEA6080817

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclone of (not in EBiSC, see SIGi001-A in hPSCreg)

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4447434

hIPSC Derivation#

General

More source cell information can be found in the parental cell line SIGi001-A in hPSCreg.

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unknown
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes

Characterisation#

No characterisation data could be found for this subclone. For more data see the parental line SIGi001-A in hPSCreg.

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
Isogenic modification
17
NM_001203252
Homozygous
an HA-tag has been added to the endogenous MAPT gene
an HA-tag has been added to the endogenous MAPT gene