SIGi001-A-14

SIGi001-A Bi-Allelic MAPT HA-tag

Gene-edited iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name SIGi001-A-14
Alternative name(s)
SIGi001-A Bi-Allelic MAPT HA-tag
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
Notes SIGi001-A having a HA-tag at the C-terminal end of MAPT, Bi-Allelic

Provider

Depositor Bioneer (BION)
Owner Bioneer (BION)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Denmark

External Databases

hPSCreg SIGi001-A-14
BioSamples SAMEA6080813
ECACC 66541213
CLO CLO_0103293
Cellosaurus CVCL_A7GS
Wikidata Q107116924

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of (not in EBiSC, see SIGi001-A in hPSCreg)

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no
Is the medical history available upon request? no
Is clinical information available? no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4447434

hIPSC Derivation#

General

More source cell information can be found in the parental cell line SIGi001-A in hPSCreg.
Passage number reprogrammed 0

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Unknown
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Stem cell marker and Tri-lineage differentiation by flow cytometry
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: +15

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
MAPT (target)
Isogenic modification
17
NM_001203252
Homozygous
an HA-tag has been added to the endogenous MAPT gene
an HA-tag has been added to the endogenous MAPT gene