SIGi001-A-16
SIGi001-A-9 Bi-Allelic MAPT HA-tag
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | SIGi001-A-16 |
Alternative name(s) |
SIGi001-A-9 Bi-Allelic MAPT HA-tag
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Notes | SIGi001-A-9 (iPSC0028 MAPT P301S+Ex10+16/Clone 7H6A1) having a HA-tag at the C-terminal end of MAPT, Bi-Allelic |
Provider |
|
Depositor | Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
hPSCreg | SIGi001-A-16 |
BioSamples | SAMEA6080815 |
ECACC | 66541215 |
CLO | CLO_0103295 |
Cellosaurus | CVCL_A7GT |
Wikidata | Q107116926 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Subclone of | (not in EBiSC, see SIGi001-A in hPSCreg) |
Donor Information#
General Donor Information |
|
Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Disease associated phenotypes |
|
Family history | no |
Is the medical history available upon request? | no |
Is clinical information available? | no |
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
BioSamples | SAMEA4447434 |
hIPSC Derivation#
General |
|
More source cell information can be found in the parental cell line
SIGi001-A in hPSCreg.
|
|
Passage number reprogrammed | +15 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? |
Unknown |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
Accutase
|
O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|||||
SOX2 |
Yes |
|||||
SSEA-4 |
Yes |
|||||
TRA 1-81 |
Yes |
|||||
SSEA-1 |
No |
Morphology pictures
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: +15
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
|