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RCi007-A

FB70c1, RCi170

iPSC line

Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
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A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name RCi007-A
Alternative name(s)
FB70c1, RCi170
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCi007-C
Donor's gene variants:
LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
RCi009-A
(Rci201, FB73c6)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
RCi005-A
(FB71c4, RCi171)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
RCi006-A
(FB69c4, RCi169)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy

Provider

Depositor Roslin Cells (RC)
Distributors
EBiSC

External Databases

hPSCreg RCi007-A
BioSamples SAMEA3961596
Cellosaurus CVCL_RF95
Wikidata Q54949510

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 55-59

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and not affected.
Synonyms
  • Facioscapulohumeral muscular dystrophy
  • Landouzy-Dejerine myopathy
  • Facioscapulohumeral myopathy
  • FSH dystrophy
  • FSHD
  • Landouzy-Dejerine dystrophy
show more synonyms
Genetic variants
LRIF1 (target)
1p13.3
Heterozygous
LRIF1: heterozygous c.1036T, c.1036C>T
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives
Has sister:
Has brother:
Has sister:

External Databases (Donor)

BioSamples SAMEA3961579

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 55-59
Passage number reprogrammed 4

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-1
No
SSEA-4
Yes
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 17
Karyotyping method: G-Banding

Other Genotyping (Cell Line)