The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.


FB71c4, RCi171

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
Timepoint: 48 hours post-passage
Magnification: 10x
Timepoint: 48 hours post-passage
Magnification: 4x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.


Cell Line

hPSCreg name RCi005-A
Alternative name(s)
FB71c4, RCi171
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.


Depositor Roslin Cells (RC)
Owner Censo an Axol Bioscience Company (CENSO)

External Databases

hPSCreg RCi005-A
BioSamples SAMEA3961534
Cellosaurus CVCL_II96
Wikidata Q54949508

General Information

This EBiSC line can be used for:
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
  • Facioscapulohumeral muscular dystrophy
  • Landouzy-Dejerine myopathy
  • Facioscapulohumeral myopathy
  • FSH dystrophy
  • FSHD
  • Landouzy-Dejerine dystrophy
show more synonyms
Genetic variants
LRIF1 (target)
LRIF1 c.1036C>T

Donor Relations

All cell lines of this donor's relatives
Has brother:
Has sister:

External Databases (Donor)

BioSamples SAMEA3961551

hIPSC Derivation#


Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 45-49

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Type of used vector free reprogramming factor(s)


Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
Passage method Enzyme-free cell dissociation
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Differentiation Potency
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No autosomal or sex chromosome aneuplodies were detected
Passage number: 27
Karyotyping method: Karyolite BoBs

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Whole genome sequencing
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee