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RCi006-A

FB69c4, RCi169

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name RCi006-A
Alternative name(s)
FB69c4, RCi169
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCi009-A
(Rci201, FB73c6)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
RCi007-A
(FB70c1, RCi170)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
RCi005-A
(FB71c4, RCi171)
Donor's gene variants:
LRIF1, LRIF1
Donor diseases:
Facioscapulohumeral dystrophy
RCi007-C
Donor's gene variants:
LRIF1
Donor diseases:
Facioscapulohumeral dystrophy

Provider

Depositor Roslin Cells (RC)
Owner Censo an Axol Bioscience Company (CENSO)
Distributors
EBiSC

External Databases

hPSCreg RCi006-A
BioSamples SAMEA3962402
Cellosaurus CVCL_EE36
Wikidata Q54949509

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 60-64

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is not a carrier of a disease-associated mutation and not affected.
Synonyms
  • Facioscapulohumeral muscular dystrophy
  • Landouzy-Dejerine myopathy
  • Facioscapulohumeral myopathy
  • FSH dystrophy
  • FSHD
  • Landouzy-Dejerine dystrophy
show more synonyms
Genetic variants
LRIF1 (target)
1p13.3
Homozygous
LRIF1: c.1036T

Donor Relations

All cell lines of this donor's relatives
Has sister:
Has sister:

External Databases (Donor)

BioSamples SAMEA3962448

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 60-64
Passage number reprogrammed 2

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
SSEA1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Yes
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
DCN
Yes
VIMENTIN
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
HES5
Yes
NEUROD1
Yes
PAX6
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 11
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768