WTSIi170-B
HPSI1213i-tolg_6
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | WTSIi170-B |
| Alternative name(s) |
HPSI1213i-tolg_6
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi170-B |
| BioSamples | SAMEA3974241 |
| HipSci | HPSI1213i-tolg_6 |
| Cellosaurus | CVCL_AX10 |
| ECACC | 77650314, 66540339 |
| CLO | CLO_0101165 |
| Wikidata | Q54891730 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 70-74 |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2398572 |
| HipSci | HPSI-tolg |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
| Age of donor (at collection) | 70-74 |
| Collected in | 2015 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 2 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
| Pluripotency Score | Novelty Score | |
| 33.73 | 1.288 |
Report
HPSI-tolg.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-tolg.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI1213i-tolg_6 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 4
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
| WGS-derived disease associations |
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
arrhythmogenic right ventricular cardiomyopathy (TTN)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (TTN)
factor XIII, b subunit, deficiency of (F13B)
Fanconi anemia complementation group A (FANCA)
Huntington disease (HTT)
hypertrophic cardiomyopathy (TTN)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 1 (IDUA)
myopathy, myofibrillar, 9, with early respiratory failure (TTN)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (MYO7A, STRC, TMPRSS3)
PHARC syndrome (ABHD12)
platelet-type bleeding disorder 18 (RASGRP2)
primary ciliary dyskinesia 5 (HYDIN)
primary ciliary dyskinesia 7 (DNAH11)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
tibial muscular dystrophy (TTN)
TTN-related myopathy (TTN)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
|
| Other WGS-derived genes | A2M, AGAP1, AGAP6, AGL, ALDH3A2, ANO7, ASCC1, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, COLQ, COQ2, CST3, CYP21A2, CYP2D6, CYP2F1, CYP3A5, D2HGDH, DHCR24, DSC3, FUT2, GALNT3, GGN, GLYCTK, GPRIN1, HLA-DRB5, HSD17B13, IRF5, ITGB2, KCNJ16, KISS1, KLHL3, LAMA5, MICA, MROH8, MST1R, MTTP, NBPF1, NDUFB9, NFU1, NPRL3, OAS1, OR1B1, OR52B4, P2RX5, PCDHB7, PDE4DIP, PIGN, POLDIP2, POM121, PRKRA, PTCHD3, RXFP2, SIGLEC12, SLC37A4, SPATA7, TBP, TIGD6, TMEM175, TMEM216, TMPRSS6, TMX3, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF233 |