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WTSIi170-A

HPSI1213i-tolg_4

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi170-A
Alternative name(s)
HPSI1213i-tolg_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi170-B
(HPSI1213i-tolg_6)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi170-A
BioSamples SAMEA3974244
HipSci HPSI1213i-tolg_4
Cellosaurus CVCL_EE40
Wikidata Q54891729

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 70-74
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398572
HipSci HPSI-tolg

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 70-74
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
33.55 1.27

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI1213i-tolg_4
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 4
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560569
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629721
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376204
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860337
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447946
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368881
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859473
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859377
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629731
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860145
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860241
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859857
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560699
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859185
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859281
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859089
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369173
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560683
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376063
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859761
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859665
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859569
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
arrhythmogenic right ventricular cardiomyopathy (TTN)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (TTN)
factor XIII, b subunit, deficiency of (F13B)
Fanconi anemia complementation group A (FANCA)
Huntington disease (HTT)
hypertrophic cardiomyopathy (TTN)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 1 (IDUA)
myopathy, myofibrillar, 9, with early respiratory failure (TTN)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (MYO7A, TMPRSS3)
PHARC syndrome (ABHD12)
platelet-type bleeding disorder 18 (RASGRP2)
primary ciliary dyskinesia 5 (HYDIN)
primary ciliary dyskinesia 7 (DNAH11)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
tibial muscular dystrophy (TTN)
TTN-related myopathy (TTN)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
A2M, AGAP1, AGAP6, AGL, ALDH3A2, ANO7, ASCC1, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, COLQ, COQ2, CST3, CYP21A2, CYP2D6, CYP2F1, CYP3A5, D2HGDH, DSC3, FUT2, GALNT3, GGN, GLYCTK, GPRIN1, HLA-DRB5, HSD17B13, IRF5, ITGB2, KCNJ16, KISS1, KLHL3, LAMA5, MICA, MROH8, MST1R, MTTP, NDUFB9, NFU1, NPRL3, OAS1, OR1B1, OR52B4, P2RX5, PCDHB7, PDE4DIP, PIGN, POLDIP2, POM121, PRKRA, PTCHD3, RXFP2, SIGLEC12, SLC37A4, SPATA7, TBP, TIGD6, TMEM175, TMEM216, TMPRSS6, TMX3, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF233