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WTSIi169-B

HPSI0614i-miaj_4

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi169-B
Alternative name(s)
HPSI0614i-miaj_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi169-A
(HPSI0614i-miaj_6)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi169-B
BioSamples SAMEA3854313
HipSci HPSI0614i-miaj_4
Cellosaurus CVCL_EE39
Wikidata Q54891148

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 50-54
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3113942
HipSci HPSI-miaj

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 50-54
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
25.953 1.452

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0614i-miaj_4
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560631
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376258
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859872
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860160
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859776
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448114
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629684
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368777
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560533
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560615
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859584
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859488
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859680
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859104
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859200
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376117
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860352
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860256
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369069
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629694
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859296
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859392
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Brugada syndrome 1 (CACNB2)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial thoracic aortic aneurysm and aortic dissection (MFAP5)
Fanconi anemia complementation group A (FANCA)
hearing loss, autosomal recessive (GJB2)
hypertrophic cardiomyopathy (CACNB2)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
methylcobalamin deficiency type cblG (MTR)
microcephaly, seizures, and developmental delay (PNKP)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (MYO7A)
obsolete syndromic genetic hearing loss (GJB2)
PHARC syndrome (ABHD12)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
short QT syndrome (CACNB2)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
AGL, ALDH3A2, AMPD1, ANAPC1, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, CPA4, CST3, CYP3A5, CYP4B1, D2HGDH, DEFB126, DNAAF1, DSC3, ERCC6L2, FANCM, FBXO7, FCGR1A, FCGR2A, FZD6, GALNT3, GLYCTK, IL12RB1, KCNJ16, KCTD18, LAMA5, LPL, MAPT, MESP1, MROH8, NDUFB9, NFU1, NOTCH2, NPRL3, OAS1, OPRM1, OR1B1, OTOR, P2RX5, PDE4DIP, PIGN, PNPLA1, POLDIP2, PTCHD3, RNF212, SIGLEC12, SLC37A4, SPATA7, SYNE2, TIGD6, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, WDR37, ZAN