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WTSIi169-A

HPSI0614i-miaj_6

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name WTSIi169-A
Alternative name(s)
HPSI0614i-miaj_6
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi169-B
(HPSI0614i-miaj_4)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi169-A
BioSamples SAMEA3962387
HipSci HPSI0614i-miaj_6
Cellosaurus CVCL_AX05
Wikidata Q54891149

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 50-54
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3113942
HipSci HPSI-miaj

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 50-54
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
22.747 1.229

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0614i-miaj_6
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 0
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369156
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629695
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860894
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860990
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860798
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860414
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860510
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448102
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560632
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376252
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861086
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861182
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368864
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859934
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560616
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629685
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560534
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860606
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860702
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376111
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861278
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860030
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, LAMC3, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial thoracic aortic aneurysm and aortic dissection (MFAP5)
Fanconi anemia complementation group A (FANCA)
hearing loss, autosomal recessive (GJB2)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
microcephaly, seizures, and developmental delay (PNKP)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (MYO7A)
obsolete syndromic genetic hearing loss (GJB2)
PHARC syndrome (ABHD12)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
AGL, ALDH3A2, AMPD1, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, CPA4, CST3, CYP3A5, CYP4B1, D2HGDH, DEFB126, DNAAF1, DSC3, ERCC6L2, FANCM, FBXO7, FCGR1A, FCGR2A, FZD6, GALNT3, GLYCTK, IL12RB1, KCNJ16, KCTD18, LAMA5, LPL, MAPT, MESP1, MROH8, NDUFB9, NFU1, NOTCH2, NPRL3, NXF2, OAS1, OPRM1, OR1B1, OTOR, P2RX5, PDE4DIP, PIGN, PNPLA1, POLDIP2, PTCHD3, RNF212, SIGLEC12, SLC37A4, SPATA7, SYNE2, TIGD6, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, WDR37, ZAN