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UOXFi007-C

MK002-7

iPSC line

The cell line has been recalled.

General#

Cell Line

hPSCreg Name UOXFi007-C
Alternative name(s)
MK002-7
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Oxford (UOXF)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg UOXFi007-C
BioSamples SAMEA103988271
Cellosaurus CVCL_LE39
Wikidata Q54991456

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson disease
The donor is affected.
Genetic variants
LRRK2 (target)
12q12
NM_198578.3(LRRK2):c.6055G>A
NP_940980.3:p.(Gly2019Ser)
Heterozygous

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives
Has daughter:

External Databases (Donor)

BioSamples SAMEA103887563

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 70-74
Collected in 2011
Passage number reprogrammed 7

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Supplements
ROCK inhibitor Y27632 10µM final concentration µM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
Pluripotency Score Novelty Score
40.77 1.2
Morphology pictures

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 10
Karyotyping method: SNP analysis Illumina Human CytoSNP-12v2.1 beadchiparray

Other Genotyping (Cell Line)