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STBCi005-C

SFC833-03-14

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name STBCi005-C
Alternative name(s)
SFC833-03-14
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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Parkinson disease
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Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
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STBCi023-C
(SFC829-03-06)
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SNCA
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Parkinson disease
STBCi025-B
(SFC834-03-03)
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STBCi042-B
(SFC848-03-04)
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GBA1
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Parkinson disease
STBCi042-C
(SFC848-03-06)
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GBA1
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STBCi043-A
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STBCi043-B
(SFC120-03-04)
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Parkinson disease
STBCi040-C
(SFC029-03-01)
Donor diseases:
Parkinson disease
STBCi041-B
(SFC081-03-04)
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(SFC120-03-02)
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STBCi083-A
(SFC830-04-09)
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SNCA
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Parkinson disease
STBCi085-B
(SFC866-03-02)
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GBA1
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Parkinson disease
STBCi086-A
(SFC842-03-02)
Donor diseases:
Parkinson disease

Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi005-C
BioSamples SAMEA104615894
Cellosaurus CVCL_RB39
Wikidata Q54956331

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 80-84

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans
Genetic variants
LRRK2 (target)
12q12
NM_198578.3:c.6055G>A
NP_940980.3:p.Gly2019Ser
VCV000001940.35
LRRK2 G2019S

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104129751

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 80-84

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No abnormalities detected
Passage number: 19
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)