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UKKi007-B

NP0014-5

iPSC line

In Stock
At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: 48 hours post-passage
Magnification: 4x
Timepoint: 48 hours post-passage
Magnification: 10x
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg Name UKKi007-B
Alternative name(s)
NP0014-5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider
Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine

External Databases
hPSCreg UKKi007-B
BioSamples SAMEA2698638
Cellosaurus CVCL_9S36
ECACC 66540013
CLO CLO_0101549
Wikidata Q54990423

General Information
Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information
Sex female
Age of donor (at collection) 45-49
Ethnicity Caucasian, German

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Catecholaminergic polymorphic ventricular tachycardia
Catecholaminergic polymorphic ventricular tachycardia in the OLS
CPVT1, Mutation in RYR2 gene c.7447T>A, p.F2483I
The donor is a carrier of a disease-associated mutation and affected.
Stage
Symptomatic
Synonyms
  • CPVT
  • Malignant paroxysmal ventricular tachycardia
  • Bidirectional ventricular tachycardia induced by catecholamine
  • Polymorphic ventricular tachycardia induced by catecholamines
show more synonyms
Genetic variants
RYR2 (target)
1q43
NM_001035.3:c.7447T>A
NP_001026.2:p.F2483I
Heterozygous
22178870
Genomic DNA sequencing confirmed the presence of this mutation in this cell line
Disease associated phenotypes
  • Catecholaminergic polymorphic ventricular tachycardia (CPVT) type 1 [CPVT1, point mutation p.F2483I, c7447T>A (Phe > Ile) in RYR2 gene]
Non-disease associated phenotypes
  • Cardiac arrhythmia at exercise; normal ECG at rest
Family history de novo mutation
Is the medical history available upon request? No (can be retreived upon request)
Is clinical information available? Not available

Karyotyping (Donor)
Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA2629512

hIPSC Derivation#

General
Source cell type
fibroblast of dermis
fibroblast of dermis in the OLS
Source cell origin
zone of skin
zone of skin in the OLS
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 45-49
Collected in 2009
Passage number reprogrammed P3

Reprogramming method
Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR
Vector map
pMX-reprogramming_vector_maps.pdf

Vector free reprogramming
Type of used vector free reprogramming factor(s)
None

Other
Selection criteria for clones human ESC morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch
Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA1-80
Yes
Morphology pictures
Brighfield image of NP0014-5 colony at day 3 post-thaw at higher mahnification; 32x objective
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
VIMENTIN
Yes
BMP4
Yes
GATA4
No
DCN
Yes
PECAM1 (CD31)
Yes
PDGF
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
No
GSC
Yes
GATA6
Yes
CXCR4
Yes
PITX1
Yes
MYH6, alpha myosin heavy chain
Yes
MLC2v, myosin light chain 2v
Yes
ACTN2, cardiac alpha actinin
Yes
RYR2
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
Yes
B-TUBULIN
Yes
NEUROD1
Yes
HES5
Yes
FOXG1
No

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 34
Karyotyping method: Molecular karyotyping using using OmniExpress Exome Chip

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes
SNP-genotyping using OmniExpress Exome Chip