UKKi007-B
NP0014-5
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | UKKi007-B |
Alternative name(s) |
NP0014-5
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
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Depositor | Klinikum der Universität zu Köln (UKK) |
Owner | Institute for Neurophysiology, Medical Faculty |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine
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External Databases |
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hPSCreg | UKKi007-B |
BioSamples | SAMEA2698638 |
Cellosaurus | CVCL_9S36 |
ECACC | 66540013 |
CLO | CLO_0101549 |
Wikidata | Q54990423 |
General Information |
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Publications |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 45-49 |
Ethnicity | Caucasian, German |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Non-disease associated phenotypes |
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Family history | de novo mutation |
Is the medical history available upon request? | No (can be retreived upon request) |
Is clinical information available? | Not available |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA2629512 |
hIPSC Derivation#
General |
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Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
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Age of donor (at collection) | 45-49 |
Collected in | 2009 |
Passage number reprogrammed | P3 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? |
Yes |
Methods used |
RT-PCR
|
Vector map | |
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | human ESC morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | Essential E8 |
Passage method | EDTA |
Surface coating | Vitronectin |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA1-80 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
HAND1 |
Yes |
VIMENTIN |
Yes |
BMP4 |
Yes |
GATA4 |
No |
DCN |
Yes |
PECAM1 (CD31) |
Yes |
PDGF |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
SOX17 |
Yes |
FOXA2 |
No |
GSC |
Yes |
GATA6 |
Yes |
CXCR4 |
Yes |
PITX1 |
Yes |
MYH6, alpha myosin heavy chain |
Yes |
MLC2v, myosin light chain 2v |
Yes |
ACTN2, cardiac alpha actinin |
Yes |
RYR2 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
PAX6 |
Yes |
Sox1 |
Yes |
B-TUBULIN |
Yes |
NEUROD1 |
Yes |
HES5 |
Yes |
FOXG1 |
No |
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Not done |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46,XX
Passage number: 34
Karyotyping method:
Molecular karyotyping using using OmniExpress Exome Chip
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
SNP-genotyping using OmniExpress Exome Chip
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