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UKKi007-A

NP0014-6, UKK007Ai

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: 48 hours post-passage
Magnification: 4x
Timepoint: 48 hours post-passage
Magnification: 10x
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name UKKi007-A
Alternative name(s)
NP0014-6, UKK007Ai
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institute for Neurophysiology, Medical Faculty
Scottish Centre for Regenerative Medicine

External Databases

hPSCreg UKKi007-A
BioSamples SAMEA2629464
Cellosaurus CVCL_2Z94
ECACC 66540001
CLO CLO_0101548
Wikidata Q54990422

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49
Ethnicity Caucasian, German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Catecholaminergic polymorphic ventricular tachycardia
CPVT1, Mutation in RYR2 gene c.7447T>A, p.F2483I
The donor is a carrier of a disease-associated mutation and affected.
Stage
Symptomatic
Synonyms
  • CPVT
  • Malignant paroxysmal ventricular tachycardia
  • Bidirectional ventricular tachycardia induced by catecholamine
  • Polymorphic ventricular tachycardia induced by catecholamines
show more synonyms
Genetic variants
RYR2 (target)
1q43
NM_001035.3:c.7447T>A
NP_001026.2:p.F2483I
Heterozygous
22178870
Genomic DNA sequencing confirmed the presence of this mutation in this cell line
Disease associated phenotypes
  • Catecholaminergic polymorphic ventricular tachycardia (CPVT) type 1 [CPVT1, point mutation p.F2483I, c7447T>A (Phe > Ile) in RYR2 gene]
  • Cardiac arrhythmia at exercise; normal ECG at rest
Family history de novo mutation
Is the medical history available upon request? No (can be retreived upon request)
Is clinical information available? Not available

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2629512

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 45-49
Collected in 2009
Passage number reprogrammed P3

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR
Notes on reprogramming vector silencing Retrovirally encoded reprogramming factors OCT4, KLF4 and cMYC are not expressed as determined by RT-PCR. Only Sox2 seems to be expressed. at very low levels.
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones human ESC morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-80
Yes
Morphology pictures
Brightfield image of NP0014-6 iPSC colony on day 4 post-thaw at higher magnification; 32x objective
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 34
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array