UKBi007-A
LB-33-5, iLB-MJD3-33f-r5
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | UKBi007-A |
Alternative name(s) |
LB-33-5, iLB-MJD3-33f-r5
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
UKBi008-A (iLB-MJD4-34m-r1, LB-34-1) Donor's gene variants: ATXN3, ATXN3 Donor diseases: Spinocerebellar ataxia type 3 UKBi003-A (iLB-MJD1-32m-r9, LB-32-r9) Donor's gene variants: ATXN3, ATXN3 Donor diseases: Spinocerebellar ataxia type 3 |
Provider |
|
Depositor | Universitätsklinikum Bonn (UKB) |
Owner | Universitätsklinikum Bonn |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Institut für Rekonstruktive Neurobiologie
Scottish Centre for Regenerative Medicine
|
External Databases |
|
hPSCreg | UKBi007-A |
BioSamples | SAMEA2825788 |
Cellosaurus | CVCL_W574 |
ECACC | 66540011 |
CLO | CLO_0101564 |
Wikidata | Q54905793 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
Sex | female |
Age of donor (at collection) | 40-44 |
Ethnicity | Caucasian, German |
Phenotype and Disease related information (Donor) |
|
Diseases | A disease was diagnosed.
|
Family history | de novo mutation |
External Databases (Donor) |
|
BioSamples | SAMEA2825825 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 40-44 |
Collected in | 2008 |
Passage number reprogrammed | P4 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Yes |
Reprogramming vectors silenced? |
Yes |
Methods used |
PCR
|
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
Culture medium | mTeSR |
Passage method | EDTA |
Surface coating | Matrigel / Geltrex |
O2 concentration | 21 |
CO2 concentration | 5 |
Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|||||
SSEA-3 |
Yes |
|||||
TRA 1-60 |
Yes |
|||||
TRA 1-81 |
Yes |
Differentiation Potency
In vivo teratoma
In vitro directed differentiation
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
|