RCi004-A-1

RCi004-A + HTT GC #H34-32_T34-23

Gene-edited iPSC line

The cell line is not available.

General#

Cell Line

hPSCreg Name RCi004-A-1
Alternative name(s)
RCi004-A + HTT GC #H34-32_T34-23
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Bioneer (BION)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg RCi004-A-1
BioSamples SAMEA7462886

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Huntington disease
The donor is a carrier of a disease-associated mutation and not affected.
Stage
Pre-symptomatic at time of biopsy
Synonyms
  • Huntington chorea
Genetic variants
HTT (target)
4p16.3
NM_002111.7:c.52CAG[40]
Heterozygous
SCV000054533.1
DNA sample indicates one CAG repeat allele within the normal range (approximately 18 repeats) and one allele within the Huntington disease affected range (approximately 40 repeats) in the HTT gene.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3106020

hIPSC Derivation#

General

The source cell information can be found in the parental cell line RCi004-A.
Passage number reprogrammed 48

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

No characterisation data could be found for this subclone. Please open parental cell line RCi004-A .

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
Huntington Disease
Genetic modifications
Isogenic modification
HTT
Heterozygous
The original line is associated with Huntington's disease and has 20 CAGs on one allele and 40 on the other. The corrected line has 17 CAGs on one allele and 25 on the other allele. Note that there is also a difference in a proline repeat next to the CAG repeat: The original line has 14 prolines on the allele with the 20 CAGs and 11 prolines on the allele with the 40 CAGs. The corrected line has 14 prolines on the allele with the 17 CAGs and 11 prolines on the allele with the 25 CAGs