CHDIi034-A

#105c1, CHDI-90002182

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name CHDIi034-A
Alternative name(s)
#105c1, CHDI-90002182
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider

Depositor CHDI Foundation Inc. (CHDI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg CHDIi034-A
BioSamples SAMEA4675663
ECACC 66541181
Cellosaurus CVCL_VD05
CLO CLO_0102311
Wikidata Q54811801

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Huntington disease
The donor is a carrier of a disease-associated mutation and affected.
Stage
Early symptomatic at time of biopsy
Synonyms
  • Huntington chorea
Genetic variants
HTT (target)
4p16.3
NM_002111.8:c.53AGC[50]AAC[1]AGC[1]
Heterozygous
One allele with 22 CAG repeats and one allele with 50 CAG repeats in HTT gene

External Databases (Donor)

BioSamples SAMEA4675664

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 45-49
Passage number reprogrammed P5

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Clone morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR1
Passage method EDTA
Surface coating Matrigel
O2 concentration 21
CO2 concentration 5
Temperature 37 °C
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
Morphology pictures
24hr post thaw x4
24hr post thaw x10
Confluency post thaw x4
Confluency post thaw x10
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
GATA6
Yes
CXCR4
Yes
Gata6
Yes
CXCR4
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
VIMENTIN
Yes
NCAM1
Yes
MIXL1
Yes
DCN
No
VIM
Yes
MIXL1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
NEUROD1
Yes
HES5
Yes
NEUROD1
Yes
PAX6
Yes
HES5
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: P15
Karyotyping method: G-Banding

Other Genotyping (Cell Line)