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RCi004-A

PDSC-10, RCi150

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name RCi004-A
Alternative name(s)
PDSC-10, RCi150
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
RCi004-B
(RCi68, PDSC-3)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi001-A
(#1c8, CHDI-90002149)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi003-A
(#3c1, CHDI-90002151)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi005-A
(#5c4, CHDI-90002153)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi020-A
(#20c2, CHDI-90002168)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi022-A
(#22c1, CHDI-90002170)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi034-A
(#105c1, CHDI-90002182)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi055-A
(#133c5, CHDI-90002203)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi002-A
(#2c3, CHDI-90002150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi006-A
(#6c7, CHDI-90002154)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi008-A
(#8c3, CHDI-90002156)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi011-A
(#11c2, CHDI-90002159)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi017-A
(#17c1, CHDI-90002165)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi018-A
(#18c1, CHDI-90002166)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi019-A
(#19c9, CHDI-90002167)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi021-A
(#21c5, CHDI-90002169)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi010-A
(#10c2, CHDI-90002158)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi026-A
(#26c3, CHDI-90002174)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi029-A
(#29c4, CHDI-90002177)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi031-A
(#31c1, CHDI-90002179)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi033-A
(#104c2, CHDI-90002181)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi041-A
(#116c2, CHDI-90002189)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi048-A
(#125c2, CHDI-90002196)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi049-A
(#127c2, CHDI-90002197)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi028-A
(#28c3, CHDI-90002176)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi035-A
(#108c5, CHDI-90002183)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi036-A
(#110c5, CHDI-90002184)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi038-A
(#112c7, CHDI-90002186)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi040-A
(#115c6, CHDI-90002188)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi045-A
(#121c6, CHDI-90002193)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CHDIi046-A
(#122c1, CHDI-90002194)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease

Provider

Depositor Roslin Cells (RC)
Owner Censo an Axol Bioscience Company (CENSO)
Distributors
EBiSC

External Databases

hPSCreg RCi004-A
BioSamples SAMEA3106011
Cellosaurus CVCL_9S47
Wikidata Q54949504

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and not affected.
Stage
Pre-symptomatic at time of biopsy
Synonyms
  • Huntington chorea
Genetic variants
HTT (target)
4p16.3
NM_002111.7:c.52CAG[40]
Heterozygous
SCV000054533.1
DNA sample indicates one CAG repeat allele within the normal range (approximately 18 repeats) and one allele within the Huntington disease affected range (approximately 40 repeats) in the HTT gene.
Disease associated phenotypes
  • Mutant gene: HTT

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3106020

hIPSC Derivation#

General

Source cell type
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 45-49
Passage number reprogrammed P4

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
GSC
Yes
CXCR4
Yes
PITX1
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
VIMENTIN
Yes
BMP4
Yes
GATA4
Yes
DCN
Yes
PECAM1 (CD31)
Yes
PDGF
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
Sox1
Yes
B-TUBULIN
No
NEUROD1
Yes
HES5
Yes
FOXG1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX,i(20)(q10)
Passage number: P16
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768