The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via Contact@EBiSC.org if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.

ESi001-A

SPO2#1

iPSC line

Available directly from depositor
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
You will need to complete a Cell Line Information Pack (CLIP) before purchasing your cell line. Please contact us to receive the relevant document.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name ESi001-A
Alternative name(s)
SPO2#1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
EDi001-A-1
(AST22-C, AST23-C)
Donor's gene variants:
SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-2
(AST23-1KO-3, AST22-1KO-3, AST-23_SCAKO Clone 3, AST-22_SNCAKO Clone 3)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
EDi001-A-3
(AST23_SNCAKO Clone 1, AST22-1KO-1, AST23-1KO-1, AST22_SNCAKO Clone 1)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
STBCi004-B-1
(SFC832-03-06 LRRK2WT/WT C47)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi002-B
(MK082-30)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi003-A
(MK088-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi267-A
(SFC027-03-02)
Donor diseases:
Parkinson disease
STBCi023-B
(SFC829-03-04)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi025-C
(SFC834-03-10)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi320-A
(SFC031-03-03)
Donor diseases:
Parkinson disease
STBCi005-A
(SFC833-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-A
(SFC855-03-06)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi085-A
(SFC866-03-06)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi083-B
(SFC830-04-08)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi088-A
(SFC872-03-05)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
EDi001-A
(AST22, AST23, SAMEA3319992)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
UOXFi001-A
(MK071-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-B
(MK071-3)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi024-A
(SFC831-03-01)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi024-C
(SFC831-03-05)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
UOXFi007-A
(MK002-4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi008-B
(MK144-7)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi019-C
(SFC828-03-04)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi004-A
(SFC832-03-19)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi084-A
(SFC871-03-12)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi084-C
(SFC871-03-09)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
EDi008-B
(G51D-4, EDINi008-B, EDIi008-B, SAMEA3174606)
Donor's gene variants:
SNCA, SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
ESi002-A
(SP08#1)
Donor diseases:
Parkinson disease
ESi006-A
(SP13#4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi090-B
(SFC867-04-12)
Donor diseases:
Parkinson disease
STBCi005-B
(SFC833-03-05)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi005-C
(SFC833-03-14)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-B
(SFC855-03-08)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
STBCi007-C
(SFC855-03-01)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
UOXFi002-A
(MK082-26)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi003-C
(MK088-7)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi258-A
(SFC090-03-07)
Donor diseases:
Parkinson disease
STBCi259-A
(SFC032-03-07)
Donor diseases:
Parkinson disease
STBCi265-A
(SFC121-03-02)
Donor diseases:
Parkinson disease
STBCi268-A
(SFC077-03-04)
Donor diseases:
Parkinson disease
STBCi279-A
(SFC063-03-01)
Donor diseases:
Parkinson disease
STBCi281-A
(SFC167-03-04)
Donor diseases:
Parkinson disease
STBCi282-A
(SFC138-03-05)
Donor diseases:
Parkinson disease
STBCi023-A
(SFC829-03-02)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi023-C
(SFC829-03-06)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
STBCi288-A
(SFC028-03-08)
Donor diseases:
Parkinson disease
STBCi289-A
(SFC030-03-02)
Donor diseases:
Parkinson disease
STBCi025-B
(SFC834-03-03)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi290-A
(SFC069-03-01)
Donor diseases:
Parkinson disease
STBCi291-A
(SFC080-03-04)
Donor diseases:
Parkinson disease

Provider

Depositor Spanish Stem Cell Bank (ES)
Distributors
EBiSC
Derivation country Spain

External Databases

hPSCreg ESi001-A
BioSamples SAMEA3302942
Cellosaurus CVCL_V201
Wikidata Q54955397

General Information

Publications
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 55-59

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Idiopathic parkinson's disease
The donor is affected.
Synonyms
  • Parkinson disease
  • Parkinson's disease
  • paralysis agitans

External Databases (Donor)

BioSamples SAMEA3302933

hIPSC Derivation#

General

Source cell type
An epidermal cell which synthesizes keratin and undergoes a characteristic change as it moves upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.; Keratinocytes are reportedly CDw210a-negative, CDw210b-positive, CD281-positive, CD282-positive, CD285-positive, IL22Ra1-positive, Human keratinocytes are reportedly capable of secreting BD-2, BD-3, hCAP-18, CXCL1, CXCL5, CXCL8, elafin, MMP-3, NGAL, PDGF-A, S100A7, S100A8, and S100A9. Transcription factors: STAT3-positive.
Synonyms
  • keratinized cell of epidermis
  • malpighian cell
Age of donor (at collection) 55-59

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Human foreskin fibroblasts
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: KnockOut DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
Glutamax 2 mM
2-mercaptoethanol 50 µM
NEAA 1 %
bFGF 10 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
CS
Yes
SOX9
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
GFAP
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes