EDi018-B
RCi212
iPSC line
The cell line has been recalled.
General#
Cell Line |
|
| hPSCreg Name | EDi018-B |
| Alternative name(s) |
RCi212
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
| Depositor | University of Edinburgh (ED) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
| hPSCreg | EDi018-B |
| BioSamples | SAMEA4773418 |
| Cellosaurus | CVCL_LE80 |
| ECACC | 66540470 |
| Wikidata | Q54832008 |
| CLO | CLO_0104773 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Family history | From multiple affected family multiple affected by bipolar and major massive depressive disorder |
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA4772668 |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblast of dermis |
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| SSEA-1 |
No |
|
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| SSEA-4 |
Yes |
|
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| TRA 1-60 |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Not done |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
No autosomal or sex chromosome aneuploidies were detected
Passage number: 18
Karyotyping method:
BACs-on-Beads
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755 This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768 |