If the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe please get in touch via Contact@EBiSC.org.

EDi010-B

RCi138

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name EDi010-B
Alternative name(s)
RCi138
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
EDi010-A
(RCi136)
Donor diseases:
bipolar disorder
STBCi249-A
(SFC171-03-01)
Donor diseases:
bipolar disorder
STBCi167-A
(SFC174-03-01)
Donor diseases:
bipolar disorder
STBCi169-A
(SFC179-03-01)
Donor diseases:
bipolar disorder
STBCi174-A
(SFC194-03-01)
Donor diseases:
bipolar disorder
EDi018-A
(RCi211)
Donor diseases:
bipolar disorder
STBCi075-A
(SFC129-03-02)
Donor diseases:
bipolar disorder
STBCi075-B
(SFC129-03-03)
Donor diseases:
bipolar disorder
STBCi075-C
(SFC129-03-01)
Donor diseases:
bipolar disorder
STBCi081-A
(SFC170-03-01)
Donor diseases:
bipolar disorder
STBCi081-B
(SFC170-03-03)
Donor diseases:
bipolar disorder
STBCi112-A
(SFC177-03-01)
Donor diseases:
bipolar disorder
STBCi113-A
(SFC178-03-01)
Donor diseases:
bipolar disorder
STBCi118-A
(SFC037-03-01)
Donor diseases:
bipolar disorder
STBCi125-A
(SFC175-03-01)
Donor diseases:
bipolar disorder
STBCi081-C
(SFC170-03-02)
Donor diseases:
bipolar disorder
EDi011-B
(RCi140)
Donor diseases:
bipolar disorder
EDi011-C
(RCi141)
Donor diseases:
bipolar disorder
STBCi068-A
(SFC035-03-01)
Donor diseases:
bipolar disorder
STBCi068-B
(SFC035-03-02)
Donor diseases:
bipolar disorder
STBCi068-C
(SFC035-03-03)
Donor diseases:
bipolar disorder
STBCi210-A
(SFC305-03-01)
Donor diseases:
bipolar disorder

Provider

Depositor University of Edinburgh (ED)
Distributors
EBiSC

External Databases

hPSCreg EDi010-B
BioSamples SAMEA4459356
Cellosaurus CVCL_LE58
Wikidata Q54831986

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Bipolar Disorder, type I
The donor is affected.
Synonyms
  • bipolar disorder
  • major affective disorder
  • manic-depression
  • bipolar depression
  • bipolar disorder manic phase
  • manic bipolar I disorder
  • manic bipolar affective disorder
  • manic depression
  • manic depressive disorder
  • manic disorder
  • mixed bipolar disorder
  • MAFD
  • bipolar affective disorder
  • depressive-manic psych.
  • major bipolar affective disorder
  • manic-depressive illness
  • manic-depressive psychosis
show more synonyms
Family history From multiple affected family multiple affected by bipolar and major depressive disorder

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA4459355

hIPSC Derivation#

General

Source cell type
Any skin fibroblast that is part of some dermis.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
SSEA-1
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuploidies detected
Passage number: 16
Karyotyping method: Karyolite BoBs

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768