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EDi014-B

RCi185

iPSC line

Not-for-profit fee: £1400 per vial
Not currently available
The cell line has been recalled.

General#

Cell Line
hPSCreg name EDi014-B
Alternative name(s)
RCi185
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider
Depositor University of Edinburgh (ED)
Distributors
EBiSC

External Databases
hPSCreg EDi014-B
BioSamples SAMEA4459371
Cellosaurus CVCL_LE69
Wikidata Q54831997

General Information
Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information
Sex male

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Obsolete_unipolar depression
Synonyms
  • Neuroses, Depressive
  • Emotional Depression
  • Depressive Disorders, Major
  • MAJOR DEPRESSIVE DIS
  • Neurosis, Depressive
  • Involutional Psychosis
  • Disorder, Major Depressive
  • Depression, Endogenous
  • Depressions, Endogenous
  • Involutional Depression
  • Syndromes, Depressive
  • Involutional Psychoses
  • Depression, Involutional
  • Depressions, Unipolar
  • Symptom, Depressive
  • Depressive Disorder, Major
  • Psychosis, Involutional
  • Depressions
  • Depressions, Emotional
  • Depression, Emotional
  • Depressive Symptom
  • Disorders, Major Depressive
  • Depressive Symptoms
  • Disorders, Depressive
  • Depression, Unipolar
  • DEPRESSIVE DIS
  • Depressive Disorders
  • Endogenous Depressions
  • Depressive Neurosis
  • Syndrome, Depressive
  • Disorder, Depressive
  • Symptoms, Depressive
  • Psychoses, Involutional
  • Depressive Neuroses
  • Depression, Neurotic
  • DEPRESSIVE DIS MAJOR
  • Paraphrenia, Involutional
  • Neurotic Depressions
  • Depressions, Neurotic
  • Depressive Syndromes
  • Depressive Syndrome
  • Depression
  • Depressive Disorder
  • Endogenous Depression
  • Emotional Depressions
  • Unipolar Depressions
  • major depressive disorder
  • Major Depressive Disorders
  • Neurotic Depression
show more synonyms
Anti-social behavior
Family history From multiple affected family multiple affected by bipolar and major depressive disorder

Donor Relations
Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)
BioSamples SAMEA4459370

hIPSC Derivation#

General
Source cell type
Fibroblast of dermis
Any skin fibroblast that is part of some dermis.
Source cell origin
Zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-1
No
SSEA-4
Yes
POU5F1 (OCT-4)
Yes

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuploidies were detected
Passage number: 21
Karyotyping method: Bacs-on-Beads

Other Genotyping (Cell Line)
Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768