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EDi015-C

RCi184

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name EDi015-C
Alternative name(s)
RCi184
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
EDi015-A
(RCi176)
Donor diseases:
unipolar depression
EDi015-B
(RCi183)
Donor diseases:
unipolar depression

Provider

Depositor University of Edinburgh (ED)
Distributors
EBiSC

External Databases

hPSCreg EDi015-C
BioSamples SAMEA4459376
Cellosaurus CVCL_LE72
Wikidata Q54832000

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 75-79

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Neuroses, Depressive
  • Emotional Depression
  • Depressive Disorders, Major
  • MAJOR DEPRESSIVE DIS
  • Neurosis, Depressive
  • Involutional Psychosis
  • Disorder, Major Depressive
  • Depression, Endogenous
  • Depressions, Endogenous
  • Involutional Depression
  • Syndromes, Depressive
  • Involutional Psychoses
  • Depression, Involutional
  • Depressions, Unipolar
  • Symptom, Depressive
  • Depressive Disorder, Major
  • Psychosis, Involutional
  • Depressions
  • Depressions, Emotional
  • Depression, Emotional
  • Depressive Symptom
  • Disorders, Major Depressive
  • Depressive Symptoms
  • Disorders, Depressive
  • Depression, Unipolar
  • DEPRESSIVE DIS
  • Depressive Disorders
  • Endogenous Depressions
  • Depressive Neurosis
  • Syndrome, Depressive
  • Disorder, Depressive
  • Symptoms, Depressive
  • Psychoses, Involutional
  • Depressive Neuroses
  • Depression, Neurotic
  • DEPRESSIVE DIS MAJOR
  • Paraphrenia, Involutional
  • Neurotic Depressions
  • Depressions, Neurotic
  • Depressive Syndromes
  • Depressive Syndrome
  • Depression
  • Depressive Disorder
  • Endogenous Depression
  • Emotional Depressions
  • Unipolar Depressions
  • major depressive disorder
  • Major Depressive Disorders
  • Neurotic Depression
  • major depressive disorder 1
  • major depressive disorder 2
  • major depression
  • major depressive disorder and accelerated response to antidepressant drug treatment
  • major depressive disorder, response to citalopram therapy in
  • recurrent major depression
  • single major depressive episode
  • unipolar depression
  • unipolar depression, susceptibility to
show more synonyms
Family history From multiple affected family multiple affected by bipolar and major massive depressive disorder

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives
Has nephew:

External Databases (Donor)

BioSamples SAMEA4459374

hIPSC Derivation#

General

Source cell type
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 75-79

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-4
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuploidies detected

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768