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CENSOi007-A

FB75R2c5, CENSOi255

iPSC line

Not-for-profit fee: £1400 per vial
Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: Confluence
Magnification: x4
Timepoint: Confluence
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line
hPSCreg name CENSOi007-A
Alternative name(s)
FB75R2c5, CENSOi255
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
CENSOi003-B
(FB79R2c6, CENSOi258)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi005-A
(FB76R2c5, CENSOi245)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi001-B
(FB78R2c2, CENSOi249)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi002-B
(FB74R2c4, CENSOi261)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy

Provider
Depositor Censo an Axol Bioscience Company (CENSO)
Distributors
EBiSC

External Databases
hPSCreg CENSOi007-A
BioSamples SAMEA104133814
Cellosaurus CVCL_LE90
Wikidata Q54809300

General Information
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information
Sex male
Age of donor (at collection) 10-14

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Duchenne muscular dystrophy
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DMD
  • Severe dystrophinopathy, Duchenne type
Genetic variants
DMD (target)
Xp21.2-p21.1
A deletion of exon 52 of the dystrophin gene
Disease associated phenotypes
  • Steroid therapy; Prednisolone 22.5mg (10 days on/10 days off); decline in motor abilities 2 years after starting steroid therapy; can still walk independently at time of sample collection.

Karyotyping (Donor)
Has the donor karyotype been analysed?
No

External Databases (Donor)
BioSamples SAMEA104133815

hIPSC Derivation#

General
Source cell type
Fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 10-14
Collected in 2014
Passage number reprogrammed 5

Reprogramming method
Vector type None

Vector free reprogramming
Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch
Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-1
No
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
CXCR4
Yes
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
VIM
Yes
NCAM1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
HES5
Yes
PAX6
Yes
NeuroD1
Yes

Microbiology / Virus Screening
HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility
Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuploidies were detected
Passage number: 11
Karyotyping method: KaryoLite BoBs

Other Genotyping (Cell Line)