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CENSOi005-A

FB76R2c5, CENSOi245

iPSC line

Immediately available for distribution*
*Once all legal and processing details completed
Timepoint: Confluence
Magnification: x4
Timepoint: Confluence
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name CENSOi005-A
Alternative name(s)
FB76R2c5, CENSOi245
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
CENSOi003-B
(FB79R2c6, CENSOi258)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi007-A
(FB75R2c5, CENSOi255)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi001-B
(FB78R2c2, CENSOi249)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy
CENSOi002-B
(FB74R2c4, CENSOi261)
Donor's gene variants:
DMD, DMD
Donor diseases:
Duchenne muscular dystrophy

Provider

Depositor Censo an Axol Bioscience Company (CENSO)
Distributors
EBiSC

External Databases

hPSCreg CENSOi005-A
BioSamples SAMEA104133812
Cellosaurus CVCL_LE89
Wikidata Q54809299

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 5-9

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • DMD
  • Severe dystrophinopathy, Duchenne type
Genetic variants
DMD (target)
Xp21.2-p21.1
Duplication of exons 3 to 41 of the dystrophin gene, predicted in frame

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

External Databases (Donor)

BioSamples SAMEA104133813

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 5-9
Collected in 2014
Passage number reprogrammed 5

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-4
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
GATA6
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
VIM
Yes
MIXL1
Yes
NCAM1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
NeuroD1
Yes
HES5
Yes
PAX6
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)