WTSIi203-A

HPSI0714i-oojs_1

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name WTSIi203-A
Alternative name(s)
HPSI0714i-oojs_1
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi203-A
BioSamples SAMEA3339626
HipSci HPSI0714i-oojs_1
ECACC 77650340
Cellosaurus CVCL_AG81
CLO CLO_0102033
Wikidata Q54891323

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 5-9
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic diabetes
Synonyms
  • monogenic diabetes mellitus

External Databases (Donor)

BioSamples SAMEA2663611
HipSci HPSI-oojs

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 5-9
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
27.643 1.488

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0714i-oojs_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment