WTSIi203-A

HPSI0714i-oojs_1

iPSC line

The cell line was withdrawn.

General#

Cell Line

hPSCreg Name WTSIi203-A
Alternative name(s)
HPSI0714i-oojs_1
Cell line type Human induced pluripotent stem cell (hiPSC)
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Donor diseases:
monogenic diabetes
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Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi203-A
BioSamples SAMEA3339626
HipSci HPSI0714i-oojs_1
ECACC 77650340
Cellosaurus CVCL_AG81
CLO CLO_0102033
Wikidata Q54891323

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 5-9
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
monogenic diabetes
Synonyms
  • monogenic diabetes mellitus

External Databases (Donor)

BioSamples SAMEA2663611
HipSci HPSI-oojs

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 5-9
Collected in 2015
Source cell line vendor University of Exeter Medical School

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
27.643 1.488

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0714i-oojs_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Text file with probe intensities
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Raw sequencing reads
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001149
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
mpileup variant calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003530
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003516
BWA alignment