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WTSIi188-A

HPSI1014i-kefb_1

iPSC line

No longer available
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi188-A
Alternative name(s)
HPSI1014i-kefb_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi188-A
BioSamples SAMEA3853038
HipSci HPSI1014i-kefb_1
Cellosaurus CVCL_EE67
Wikidata Q54891589

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA3107606
HipSci HPSI-kefb

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 65-69
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
25.724 1.416

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI1014i-kefb_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376075
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860572
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859900
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860476
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447984
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369094
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560592
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376216
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860956
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368802
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629725
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861148
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860380
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859996
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861244
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376406
BWA alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860764
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860668
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860860
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560608
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629735
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560526
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376582
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376758
mpileup variant calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861052
Raw sequencing reads
WGS-derived disease associations
Brugada syndrome 1 (CACNB2)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial ovarian cancer (XRCC2)
Fanconi anemia complementation group A (FANCA)
hereditary breast carcinoma (XRCC2)
hypertrophic cardiomyopathy (CACNB2)
intellectual disability (CDH15)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, TMPRSS3)
primary ciliary dyskinesia 2 (DNAAF3)
primary ciliary dyskinesia 5 (HYDIN)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
short QT syndrome (CACNB2)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
AGAP1, AGAP6, AGL, ALDH3A2, ATXN3, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, CNOT1, COLQ, CST3, CYP2F1, CYP4B1, D2HGDH, DEFB126, DNAAF1, DSC3, DUSP6, FBXO7, FCGR2A, FUT2, GLYCTK, HSD17B13, ITGB2, KCNJ16, KCNMB3, KISS1, LAMA5, MICA, MROH8, MTTP, NDUFB9, NFU1, NOTCH2, NPRL3, OAS1, OR1B1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PRKRA, PTCHD3, PYGL, RP1L1, RXFP2, SIGLEC12, SLC37A4, SPATA7, SRA1, SYNE2, TAP2, TGIF1, TIGD6, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, USP45, VDR, WDR37