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WTSIi187-A

HPSI1013i-sita_1

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name WTSIi187-A
Alternative name(s)
HPSI1013i-sita_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi187-A
BioSamples SAMEA3848359
HipSci HPSI1013i-sita_1
Cellosaurus CVCL_EE66
Wikidata Q54891568

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 40-44
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398527
HipSci HPSI-sita

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 40-44
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
22.319 1.38
Differentiation Potency
Ectoderm
Ont Id: UBERON_0000924

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI1013i-sita_1
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 2
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369118
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560679
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859890
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861042
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629753
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376141
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860658
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860754
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860850
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560695
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860370
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861138
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861234
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859986
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368826
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860946
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448175
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629744
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560565
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376282
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860466
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860562
Raw sequencing reads
WGS-derived disease associations
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
factor XIII, b subunit, deficiency of (F13B)
microphthalmia, syndromic 2 (BCOR)
mitochondrial disease (NDUFS6)
monogenic diabetes (ABCC8, PAX4)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23)
obsolete adult hypophosphatasia (ALPL)
obsolete childhood hypophosphatasia (ALPL)
obsolete infantile hypophosphatasia (ALPL)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
PTEN hamartoma tumor syndrome (PTEN)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
Other WGS-derived genes
ADAM33, AGAP6, ALDH3A2, ANAPC1, ATXN3, AURKC, BMP4, BTNL2, CARD8, CASP12, CATSPER2, CHST15, CLDN16, COLQ, CYP3A5, D2HGDH, DEFB126, DNAAF1, DSC3, ERCC6L2, FBXO7, FUT2, FZD6, GALNT3, GLYCTK, GPRIN1, HAP1, HEY1, IL12RB1, INMT, IRF5, ITGB2, KCNJ16, KISS1, LPA, LPL, MED15, MICA, MROH8, MTTP, NDUFB9, NOTCH2, NPRL3, OAS1, OR4F15, OR51F1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, PTCHD3, PYGL, RNASEL, RNF212, RXFP2, SCAPER, SIGLEC12, SLC37A4, TBP, TIGD6, TMC3, TMEM107, TMEM175, TMEM186, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, UGT2A1, VDR, WDR37, ZAN, ZNF83