The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via Contact@EBiSC.org if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.

WTSIi181-A

HPSI0514i-rutc_2

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi181-A
Alternative name(s)
HPSI0514i-rutc_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi181-A
BioSamples SAMEA3974014
HipSci HPSI0514i-rutc_2
Cellosaurus CVCL_AX03
Wikidata Q54890922

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2533335
HipSci HPSI-rutc

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 60-64
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
21.134 1.27

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0514i-rutc_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629729
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859379
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859571
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859475
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368788
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859091
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860339
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369080
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560658
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376221
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859667
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859763
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447993
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560560
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629719
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376080
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859187
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859283
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560674
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860243
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859859
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860147
Raw sequencing reads
WGS-derived disease associations
arrhythmogenic right ventricular cardiomyopathy (TTN)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA, TTN)
hypertrophic cardiomyopathy (TTN)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy, myofibrillar, 9, with early respiratory failure (TTN)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, LOXHD1)
PHARC syndrome (ABHD12)
platelet-type bleeding disorder 11 (GP6)
primary ciliary dyskinesia 7 (DNAH11)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
tibial muscular dystrophy (TTN)
TTN-related myopathy (TTN)
Usher syndrome type 1 (CDH23)
Other WGS-derived genes
AGAP1, AGAP6, AGL, ATP13A5, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHIT1, CHST15, CST3, CYP2D6, CYP2F1, CYP3A5, CYP4B1, D2HGDH, DEFB126, DSC3, DUSP6, FCGR2A, FUT2, GALNT3, GLYCTK, HLA-DRB5, HSD17B13, IDO2, IL12RB1, INMT, IRF5, ITGB2, KCNJ16, LAMA5, LPL, MAPT, MESP1, MROH8, MYH15, NBAS, NDUFB9, NFU1, NPRL3, OAS1, OR1B1, P2RX5, PADI2, PDE4DIP, PIGN, PITX2, POLDIP2, PRKRA, RNF212, SCAPER, SLC37A4, SYNE2, TDG, TGIF1, TIGD6, TMEM107, TMEM216, TMPRSS6, TNRC18, TREH, TRPM1, VDR, WDR37, ZNF141, ZNF527, ZNF83