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WTSIi180-B

HPSI0514i-puie_5

iPSC line

Not-for-profit fee: £1400 per vial
Stock not immediately available - enquire for details
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

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General#

Cell Line

hPSCreg name WTSIi180-B
Alternative name(s)
HPSI0514i-puie_5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi180-A
(HPSI0514i-puie_4)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi180-B
BioSamples SAMEA3962560
HipSci HPSI0514i-puie_5
Cellosaurus CVCL_EE59
Wikidata Q54890916

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2517315
HipSci HPSI-puie

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 60-64
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
24.232 1.357

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0514i-puie_5
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 2
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369075
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629716
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859897
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861049
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861241
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859993
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861145
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560665
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860569
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860665
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860761
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368783
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560551
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376183
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560649
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629726
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860473
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860377
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447883
GATK haplotype calls
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376042
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860857
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860953
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Fanconi anemia complementation group A (FANCA)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
PTEN hamartoma tumor syndrome (PTEN)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
Other WGS-derived genes
AGXT2, AHNAK, ALDH3A2, AMPD1, ANKRD30A, ATXN3, AURKC, BMP4, CASP12, CATSPER2, CELSR1, CHST15, CNOT1, COLQ, CYP21A2, D2HGDH, DEFB126, DSC3, DUSP6, EMILIN2, ERCC6L2, FLG2, FMO2, FUT2, GALNT3, GLYCTK, GRID1, H6PD, IDO2, IFIH1, IL12RB1, IRF5, ITGB2, KCNJ16, KISS1, KRT18, KRT83, LAMA5, MAPT, MICA, MROH8, NDUFB9, NFU1, NPRL3, OAS1, OPRM1, P2RX5, PDE4DIP, PIGN, PITX2, POLDIP2, POLR3B, PPP1R3A, PTCHD3, PYGL, SELPLG, SIGLEC12, SLC37A4, SPATA7, SRA1, SULT1C3, SYNE2, TDG, TIGD6, TMEM107, TMEM216, TMX3, TOR1AIP1, TPTE, TREH, TRPM1, VDR, VRK1, WDR37, ZAN, ZNF99