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WTSIi179-A

HPSI0314i-wigw_2

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi179-A
Alternative name(s)
HPSI0314i-wigw_2
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Owner Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi179-A
BioSamples SAMEA3969710
HipSci HPSI0314i-wigw_2
Cellosaurus CVCL_EE57
ECACC 77650315, 66540352
CLO CLO_0101179
Wikidata Q54890628

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2425049
HipSci HPSI-wigw

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 65-69
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
11.044 1.401

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0314i-wigw_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 1
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368732
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560705
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629723
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376267
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860498
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860594
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860018
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860690
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859922
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560689
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861074
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861170
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369024
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560575
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376126
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860786
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860882
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860402
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629733
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861266
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860978
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448137
GATK haplotype calls
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
CEP290-related ciliopathy (CEP290)
Charcot-Marie-Tooth disease (FIG4)
classic homocystinuria (CBS)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Fanconi anemia complementation group A (FANCA)
hearing loss, autosomal recessive (GJB2)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
methylcobalamin deficiency type cblG (MTR)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 4A (GALNS)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23)
obsolete syndromic genetic hearing loss (GJB2)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
A2M, AGAP1, AGAP6, AGL, AKR1E2, ALDH3A2, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, CLDN16, COLQ, CYP2D6, CYP4B1, D2HGDH, DEFB126, DSC3, FASTKD1, FUT2, GALNT3, GDPD4, GLYCTK, GPRC6A, GPRIN1, IL12RB1, ITGB2, KCNJ16, KLHL3, LAMA5, LPAR6, LPL, MAPT, MED15, MROH8, MTTP, NDUFB9, NOTCH2, NXF2, OAS1, OPRM1, OR1B1, P2RX5, PDE4DIP, PIGN, POLDIP2, POLQ, PTCHD3, PYGL, RP1L1, SIGLEC12, SLC37A4, SRA1, SYNE2, TAP2, TBP, TGIF1, TIGD6, TMEM107, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, ULK4, VDR, WDR37, ZAN, ZNF141, ZNF83