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WTSIi178-A

HPSI0215i-fawm_2

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi178-A
Alternative name(s)
HPSI0215i-fawm_2
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi178-A
BioSamples SAMEA3735541
HipSci HPSI0215i-fawm_2
Cellosaurus CVCL_EE55
ECACC 77650288, 66540350
CLO CLO_0101177
Wikidata Q54890559

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3249307
HipSci HPSI-fawm

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 70-74
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
24.668 1.274

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0215i-fawm_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 2
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376837
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376661
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859122
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859026
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369082
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629677
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376132
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860274
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859506
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859602
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860178
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560517
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376485
BWA alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448149
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629687
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560599
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859410
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860082
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859698
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859794
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859218
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859314
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368790
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560583
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376273
Splice-aware STAR alignment
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial ovarian cancer (GEN1)
hereditary breast carcinoma (GEN1)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (LOXHD1)
PTEN hamartoma tumor syndrome (PTEN)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Other WGS-derived genes
A2M, ACTRT1, ADAM33, AGAP1, ALDH3A2, ARMS2, ATXN3, AURKC, BMP4, CARD8, CASP12, CATSPER2, CHST15, COG2, CYP21A2, CYP2D6, DEFB126, DNAAF1, DNAH9, DSC3, DUSP6, ERCC6L2, FANCM, FBXO7, GALNT3, GDPD4, GPRIN1, HSD17B13, IL12RB1, IRF5, ITGB2, KCNJ16, LAMA5, MOCOS, MROH8, MTTP, NBPF1, NDUFB9, NPRL3, OAS1, OR1B1, OR52B4, P2RX5, PCK2, PDE4DIP, PIGN, POLDIP2, PRKRA, PTCHD3, PYGL, RNF212, SELPLG, SLC37A4, SPATA7, SRA1, SYNE2, TIGD6, TMEM107, TMEM175, TMEM216, TMPRSS6, TMX3, TNRC18, TREH, TRPM1, VDR, WDR37, ZAN, ZNF527