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WTSIi176-A

HPSI0714i-iudw_1

iPSC line

Stock not immediately available - enquire for details
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
To receive the Certificate of Analysis, please contact us.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi176-A
Alternative name(s)
HPSI0714i-iudw_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi176-B
(HPSI0714i-iudw_4)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi176-A
BioSamples SAMEA3851971
HipSci HPSI0714i-iudw_1
Cellosaurus CVCL_EE52
Wikidata Q54891300

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 50-54
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2632927
HipSci HPSI-iudw

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 50-54
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
28.04 1.411

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0714i-iudw_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369271
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376189
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376723
mpileup variant calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859444
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859540
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859348
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447897
GATK haplotype calls
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860308
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859060
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629679
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376048
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859636
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859732
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560602
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560520
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376371
BWA alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376547
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859252
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859156
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368979
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560586
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629689
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859828
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860116
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860212
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
autosomal recessive limb-girdle muscular dystrophy (SGCA)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (LAMC3)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
Fanconi anemia complementation group A (FANCA)
hereditary nonpolyposis colon cancer (XRCC4)
Leigh syndrome (HIBCH)
mandibuloacral dysplasia with type B lipodystrophy (ZMPSTE24)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (MYO7A)
Noonan syndrome (A2ML1)
obsolete lethal restrictive dermopathy (ZMPSTE24)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
Usher syndrome type 1 (MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
A2M, ABCA10, ABCB7, ADAMTSL4, AGL, ALDH3A2, ANAPC1, ATXN3, AURKC, BMP4, CARD8, CASP12, CHST15, CNOT1, COG2, CST3, CYP21A2, CYP2D6, CYP2F1, D2HGDH, DEFB126, DSC3, FANCM, FCGR2A, FLG, FZD6, GDPD4, GLYCTK, GPRC6A, HLA-DRB5, HSD17B13, IL12RB1, IRF5, ITGB2, KCNJ16, KISS1, MESP1, MROH8, MTTP, NFU1, NOTCH2, OAS1, OR1B1, OR51F1, P2RX5, PDE4DIP, PIGN, POLDIP2, POM121, PRKRA, PTCHD3, PYGL, RNASEL, RXFP2, SCAPER, SIGLEC12, SLC37A4, SPATA7, SYNE2, TCF3, TIGD6, TK2, TLR5, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF83