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WTSIi175-A

HPSI1014i-tixi_4

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi175-A
Alternative name(s)
HPSI1014i-tixi_4
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi175-A
BioSamples SAMEA3962389
HipSci HPSI1014i-tixi_4
Cellosaurus CVCL_EE51
ECACC 77650312, 66540346
CLO CLO_0101173
Wikidata Q54891603

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2821483
HipSci HPSI-tixi

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 70-74
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
11.321 1.511

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI1014i-tixi_4
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369054
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861013
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860053
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629720
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376225
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860437
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860533
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560567
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376084
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860917
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368762
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560681
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629730
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859957
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861109
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861205
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861301
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560697
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860725
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860629
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860821
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448004
GATK haplotype calls
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
Fanconi anemia complementation group A (FANCA)
Leigh syndrome (HIBCH)
microcephaly, seizures, and developmental delay (PNKP)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A, TMPRSS3)
PHARC syndrome (ABHD12)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
Other WGS-derived genes
ABCA10, AGL, ALDH3A2, ARMS2, AURKC, BMP4, C1orf141, CASP12, CHST15, CLDN16, DEFB126, DNAAF1, DSC3, ERCC6L2, FASTKD1, FBXO7, FCGR2A, FUT2, GLYCTK, GPR161, H6PD, HLA-DRB5, IDO2, IL12RB1, IRF5, ITGB2, KCNJ16, KISS1, LAMA5, LPAR6, MAPT, MESP1, MICA, MROH8, MTTP, NBPF1, NDUFB9, NFU1, OR1B1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, PRKRA, PYGL, RNF212, RP1L1, SCAPER, SELPLG, SIGLEC12, SLC37A4, SPEN, SRA1, SYNE2, TBP, TGIF1, TIGD6, TMEM107, TMEM216, TMX3, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZNF141, ZNF83