WTSIi175-A
HPSI1014i-tixi_4
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | WTSIi175-A |
Alternative name(s) |
HPSI1014i-tixi_4
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
|
Depositor | Wellcome Sanger Institute (WTSI) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
Derivation country | United Kingdom |
External Databases |
|
hPSCreg | WTSIi175-A |
BioSamples | SAMEA3962389 |
HipSci | HPSI1014i-tixi_4 |
Cellosaurus | CVCL_EE51 |
ECACC | 77650312, 66540346 |
CLO | CLO_0101173 |
Wikidata | Q54891603 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
Sex | female |
Age of donor (at collection) | 70-74 |
Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA2821483 |
HipSci | HPSI-tixi |
hIPSC Derivation#
General |
|
Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 70-74 |
Collected in | 2015 |
Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Notes on reprogramming vector detection | CytoTune 2 |
Vector free reprogramming |
|
Other |
|
Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
CO2 Concentration | 5 % |
Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
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SOX2 |
Yes |
|
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NANOG |
Yes |
|
Pluripotency Score | Novelty Score | |
11.321 | 1.511 |
Report
HPSI-tixi.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-tixi.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI1014i-tixi_4 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
WGS-derived disease associations |
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
Fanconi anemia complementation group A (FANCA)
Leigh syndrome (HIBCH)
microcephaly, seizures, and developmental delay (PNKP)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A, TMPRSS3)
PHARC syndrome (ABHD12)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
|
Other WGS-derived genes | ABCA10, AGL, ALDH3A2, ARMS2, AURKC, BMP4, C1orf141, CASP12, CHST15, CLDN16, DEFB126, DNAAF1, DSC3, ERCC6L2, FASTKD1, FBXO7, FCGR2A, FUT2, GLYCTK, GPR161, H6PD, HLA-DRB5, IDO2, IL12RB1, IRF5, ITGB2, KCNJ16, KISS1, LAMA5, LPAR6, MAPT, MESP1, MICA, MROH8, MTTP, NBPF1, NDUFB9, NFU1, OR1B1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, PRKRA, PYGL, RNF212, RP1L1, SCAPER, SELPLG, SIGLEC12, SLC37A4, SPEN, SRA1, SYNE2, TBP, TGIF1, TIGD6, TMEM107, TMEM216, TMX3, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZNF141, ZNF83 |