WTSIi173-A
HPSI0414i-naju_1
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
hPSCreg Name | WTSIi173-A |
Alternative name(s) |
HPSI0414i-naju_1
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
WTSIi002-A (HPSI0913i-eika_2) WTSIi004-A (HPSI1113i-qolg_3) WTSIi006-A (HPSI1113i-hayt_1) WTSIi008-A (HPSI1013i-kuxp_1) WTSIi010-A (HPSI0314i-xugn_1) WTSIi013-A (HPSI0214i-kucg_2) WTSIi014-A (HPSI0214i-heja_2) WTSIi016-A (HPSI0114i-oevr_3) WTSIi018-A (HPSI0114i-kolf_3) WTSIi019-A (HPSI0114i-iisa_3) WTSIi022-A (HPSI1013i-yemz_1) WTSIi023-A (HPSI0114i-zapk_3) WTSIi025-A (HPSI1013i-pamv_1) WTSIi031-A (HPSI0314i-bipt_1) WTSIi032-A (HPSI1113i-bima_1) WTSIi039-A (HPSI1013i-wopl_1) WTSIi042-A (HPSI1013i-jufd_2) WTSIi014-B (HPSI0214i-heja_1) WTSIi010-B (HPSI0314i-xugn_2) WTSIi025-B (HPSI1013i-pamv_3) WTSIi004-B (HPSI1113i-qolg_1) WTSIi022-B (HPSI1013i-yemz_3) WTSIi006-B (HPSI1113i-hayt_3) WTSIi019-B (HPSI0114i-iisa_1) WTSIi050-A (HPSI1014i-toss_3) WTSIi051-A (HPSI1113i-uofv_1) WTSIi053-A (HPSI0413i-corn_1) WTSIi054-A (HPSI0413i-nudd_1) WTSIi064-A (HPSI0613i-bohf_1) WTSIi065-A (HPSI0613i-eojr_2) WTSIi068-A (HPSI0613i-giuo_5) WTSIi070-A (HPSI0713i-terl_1) WTSIi072-A (HPSI0114i-fikt_3) WTSIi065-B (HPSI0613i-eojr_3) WTSIi070-B (HPSI0713i-terl_2) WTSIi042-B (HPSI1013i-jufd_3) WTSIi078-A (HPSI0314i-fafq_1) WTSIi079-A (HPSI1013i-hiaf_2) WTSIi081-A (HPSI1113i-qorq_2) WTSIi084-A (HPSI0713i-cicb_3) WTSIi090-A (HPSI0813i-robp_3) WTSIi091-A (HPSI0813i-ffdc_11) WTSIi092-A (HPSI0713i-nemb_1) WTSIi093-A (HPSI0413i-sukz_1) WTSIi095-A (HPSI0813i-vajx_2) WTSIi101-A (HPSI0513i-fejf_2) WTSIi106-A (HPSI0613i-hikj_2) WTSIi107-A (HPSI0813i-iill_1) WTSIi081-B (HPSI1113i-qorq_1) WTSIi084-B (HPSI0713i-cicb_2) |
Provider |
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Depositor | Wellcome Sanger Institute (WTSI) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
Derivation country | United Kingdom |
External Databases |
|
hPSCreg | WTSIi173-A |
BioSamples | SAMEA3962372 |
HipSci | HPSI0414i-naju_1 |
Cellosaurus | CVCL_EE48 |
ECACC | 77650297, 66540344 |
CLO | CLO_0101170 |
Wikidata | Q54890717 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
Sex | male |
Age of donor (at collection) | 60-64 |
Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA2438675 |
HipSci | HPSI-naju |
hIPSC Derivation#
General |
|
Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
Age of donor (at collection) | 60-64 |
Collected in | 2015 |
Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Notes on reprogramming vector detection | CytoTune 2 |
Vector free reprogramming |
|
Other |
|
Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
CO2 Concentration | 5 % |
Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
|
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SOX2 |
Yes |
|
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NANOG |
Yes |
|
Pluripotency Score | Novelty Score | |
19.98 | 1.296 |
Report
HPSI-naju.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-naju.pluritest.novelty_score.20161010.png
novelty image
Sterility |
|
Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-naju_1 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
WGS-derived disease associations |
Brugada syndrome 1 (CACNB2)
Charcot-Marie-Tooth disease (FIG4, HINT1)
ciliopathy (TMEM67)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial ovarian cancer (BRIP1)
Fanconi anemia complementation group J (BRIP1)
hereditary breast carcinoma (BRIP1)
Hermansky-Pudlak syndrome 4 (HPS4)
hyperprolinemia type 1 (PRODH)
hypertrophic cardiomyopathy (CACNB2)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
short QT syndrome (CACNB2)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
|
Other WGS-derived genes | AGAP6, AKR1E2, ARMS2, AURKC, BMP4, CARD9, CASP12, CATSPER2, CLDN16, COLQ, CST3, D2HGDH, DEFB126, DNAAF1, DSC3, ERCC6L2, FUT2, GALNT3, GLYCTK, H6PD, HSD17B13, IDO2, INMT, IRF5, ITGB2, KCNJ16, KISS1, KRT18, LAMA5, MED15, MESP1, MICA, MPO, MROH8, MTTP, NDUFB9, NFU1, NPRL3, NXF2, OAS1, OR1B1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, PTCHD3, PYGL, RNF212, SCAPER, SLC37A4, SPATA7, SRA1, SYNE2, TAP2, TDG, TGIF1, TIGD6, TMEM107, TMEM216, TMX3, TNRC18, TREH, TRPM1, VDR, WDR37, ZAN, ZNF141 |