WTSIi171-B

HPSI0914i-zerv_8

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi171-B
Alternative name(s)
HPSI0914i-zerv_8
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Wellcome Trust Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi171-B
BioSamples SAMEA4089454
HipSci HPSI0914i-zerv_8
Cellosaurus CVCL_EE45
ECACC 77650327, 66540341
CLO CLO_0101167
Wikidata Q54891523

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 40-44
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2784229
HipSci HPSI-zerv

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 40-44
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
33.726 1.258

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0914i-zerv_8
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369219
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860645
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860549
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860741
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629741
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376195
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859877
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861221
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861125
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861029
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859973
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680154
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680250
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376553
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376729
mpileup variant calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860933
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860837
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447914
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368927
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629750
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680186
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1680218
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376054
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860357
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860453
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ376377
BWA alignment
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
alkaptonuria (HGD)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Fanconi anemia complementation group A (FANCA)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (ABCC8, PAX4)
mucopolysaccharidosis type 4A (GALNS)
multiple acyl-CoA dehydrogenase deficiency (ETFB)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, LOXHD1)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 5 (HYDIN)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
AGAP1, AGAP6, AGL, ALDH3A2, AMPD1, ARMS2, ATXN3, AURKC, BMP4, C4orf47, CARD8, CASP12, CATSPER2, CHST15, COG2, COLQ, CST3, CYP2F1, D2HGDH, DEFB126, DSC3, ERCC6L2, FASTKD1, FBXO7, FLG2, FZD6, GALNT3, GDPD4, GLYCTK, HSD17B13, IDO2, IRF5, ITGB2, KCNJ16, LAMA5, MAPT, MESP1, MICA, MROH8, NDUFB9, NFU1, OAS1, OPRM1, OR1B1, OR51F1, OR52B4, OTOR, PDE4DIP, PIGN, PITX2, POLDIP2, POLR3B, PRKRA, PTCHD3, RNF212, RXFP2, SIGLEC12, SLC37A4, SRA1, TIGD6, TMEM107, TMEM216, TMPRSS6, TNRC18, TREH, TRPM1, VDR, VRK1, WDR37, ZAN, ZNF233, ZNF99