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WTSIi168-B

HPSI0214i-poih_2

iPSC line

Not-for-profit fee: £1400 per vial
No longer available
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi168-B
Alternative name(s)
HPSI0214i-poih_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi168-A
(HPSI0214i-poih_4)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi168-B
BioSamples SAMEA3968761
HipSci HPSI0214i-poih_2
Cellosaurus CVCL_EE42
Wikidata Q54890551

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398019
HipSci HPSI-poih

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 70-74
Collected in 2015
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score
23.984 1.195

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0214i-poih_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 4
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859236
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859332
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448196
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ369149
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560645
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629713
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376150
Abundances of transcripts
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860292
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859428
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859620
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859524
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860196
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ368857
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560547
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376291
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859140
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859044
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1560661
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629703
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860100
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859812
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859716
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4)
classic homocystinuria (CBS)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
factor XIII, b subunit, deficiency of (F13B)
Fanconi anemia complementation group A (FANCA)
focal segmental glomerulosclerosis 9 (CRB2)
Huntington disease (HTT)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (ABCC8, PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, SYNE4)
PHARC syndrome (ABHD12)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
AGL, ALDH3A2, ANAPC1, ARMS2, AURKC, BMP4, CARD8, CASP12, CEP170, CHST15, CNOT1, COG2, COQ2, CRYBA4, CST3, CYP2F1, CYP4B1, DEFB126, DNAAF1, DNAJA4, DSC3, DUSP6, FBXO7, GALNT3, GLYCTK, GPRIN1, GRID1, HLA-DRB5, HSD17B13, IRF5, ITGB2, KCNJ16, KISS1, LAMA5, MESP1, MICA, MROH8, MTTP, NDUFB9, NPRL3, OAS1, OR1B1, P2RX5, PDE4DIP, PIGN, PITX2, POLDIP2, PRKRA, RNF212, SCAPER, SELPLG, SIGLEC12, SLC37A4, SRA1, SYNE2, TBP, TGIF1, TIGD6, TMEM107, TMEM175, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, UGT1A10, UGT2A1, VDR, WDR37, ZAN, ZNF141