WTSIi144-A
HPSI0114i-wegi_1
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | WTSIi144-A |
| Alternative name(s) |
HPSI0114i-wegi_1
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi067-A (HPSI0713i-foop_1) WTSIi067-B (HPSI0713i-foop_2) WTSIi071-A (HPSI0913i-lise_1) WTSIi066-B (HPSI0613i-febc_2) WTSIi012-B (HPSI0314i-bubh_3) WTSIi074-A (HPSI0114i-joxm_1) WTSIi017-B (HPSI0114i-lexy_1) WTSIi075-A (HPSI0114i-vass_1) WTSIi076-A (HPSI0214i-kehc_2) WTSIi077-A (HPSI0314i-cuhk_1) WTSIi011-B (HPSI0314i-qonc_1) WTSIi003-A (HPSI1213i-hehd_2) WTSIi005-A (HPSI1113i-podx_1) WTSIi007-A (HPSI1113i-dons_1) WTSIi009-A (HPSI0913i-diku_1) WTSIi011-A (HPSI0314i-qonc_2) WTSIi012-A (HPSI0314i-bubh_1) WTSIi015-A (HPSI0114i-rozh_5) WTSIi020-A (HPSI0114i-eipl_1) WTSIi021-A (HPSI1013i-wuye_2) WTSIi024-A (HPSI0114i-vabj_3) WTSIi026-A (HPSI0314i-hoik_1) WTSIi027-A (HPSI0913i-oapg_5) WTSIi028-A (HPSI1213i-babk_2) WTSIi029-A (HPSI1013i-garx_2) WTSIi030-A (HPSI0214i-pelm_3) WTSIi040-A (HPSI0314i-qaqx_1) WTSIi041-A (HPSI0114i-bezi_1) WTSIi015-B (HPSI0114i-rozh_4) WTSIi021-B (HPSI1013i-wuye_3) WTSIi044-A (HPSI0214i-eiwy_1) WTSIi045-A (HPSI0214i-heth_1) WTSIi005-B (HPSI1113i-podx_2) WTSIi046-A (HPSI0214i-wibj_2) WTSIi047-A (HPSI0814i-bokz_5) WTSIi038-B (HPSI1113i-ieki_2) WTSIi048-A (HPSI0214i-datg_2) WTSIi047-B (HPSI0814i-bokz_6) WTSIi049-A (HPSI0514i-wiii_2) WTSIi030-B (HPSI0214i-pelm_1) WTSIi049-B (HPSI0514i-wiii_3) WTSIi052-A (HPSI1114i-ziyn_6) WTSIi055-A (HPSI0513i-debk_9) WTSIi056-A (HPSI0513i-dipe_1) WTSIi057-A (HPSI0513i-giuf_3) WTSIi058-A (HPSI0513i-suzg_3) WTSIi060-A (HPSI0513i-veqz_6) WTSIi061-A (HPSI0513i-veve_2) WTSIi062-A (HPSI0513i-zaui_3) WTSIi066-A (HPSI0613i-febc_1) |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi144-A |
| BioSamples | SAMEA2547631 |
| HipSci | HPSI0114i-wegi_1 |
| Cellosaurus | CVCL_AE38 |
| ECACC | 77650197, 66540254 |
| CLO | CLO_0101157 |
| Wikidata | Q54890454 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
| Sex | female |
| Age of donor (at collection) | 60-64 |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA2397971 |
| HipSci | HPSI-wegi |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
| Age of donor (at collection) | 60-64 |
| Collected in | 2014 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
| Pluripotency Score | Novelty Score | |
| 34.262 | 1.317 |
Report
HPSI-wegi.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-wegi.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0114i-wegi_1 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1 |
| WGS-derived disease associations |
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Brugada syndrome 1 (CACNB2)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
factor XIII, b subunit, deficiency of (F13B)
Hermansky-Pudlak syndrome 4 (HPS4)
hypertrophic cardiomyopathy (CACNB2)
Leigh syndrome (HIBCH)
microcephaly, seizures, and developmental delay (PNKP)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
short QT syndrome (CACNB2)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (ZNF711)
|
| Other WGS-derived genes | A2M, ABCA10, AGL, AHNAK, ALDH3A2, ANAPC1, AURKC, BMP4, CARD8, CASP12, CHST15, COLQ, CST3, CTC1, CYP21A2, CYP2D6, DEFB126, DSC3, ERCC6L2, FAM151A, FBXO7, FZD6, GALNT3, GDPD4, GLYCTK, H6PD, INMT, IRF5, ITGB2, KCNJ16, KISS1, LAMA5, LPAR6, MESP1, MROH8, MTTP, NDUFB9, NFU1, NOTCH2, NRP2, OAS1, OR1B1, OR51F1, P2RX5, PDE4DIP, PIGN, POLDIP2, PRKRA, PYGL, RNF212, SCAPER, SIGLEC12, SLC37A4, SPATA7, SRA1, SYNE2, TGIF1, TIGD6, TLR5, TMEM107, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRMT10B, TRPM1, USP45, VDR, WDR37, ZNF844 |