WTSIi139-A

HPSI1113i-oaaz_2

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi139-A
Alternative name(s)
HPSI1113i-oaaz_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi139-B
(HPSI1113i-oaaz_3)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi139-A
BioSamples SAMEA2459970
HipSci HPSI1113i-oaaz_2
Cellosaurus CVCL_AH97
ECACC 77650187, 66540248
CLO CLO_0101148
Wikidata Q54891648

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 70-74
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2399135
HipSci HPSI-oaaz

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 70-74
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
46.691 1.339 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1113i-oaaz_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 5
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279087
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279083
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861347
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266992
Abundances of transcripts
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127459
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127694
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1274916
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1279065
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447934
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ266948
Splice-aware STAR alignment
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1274908
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266681
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266775
Imputed and phased genotypes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ266871
BWA alignment
WGS-derived disease associations
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
collagen 6-related myopathy (COL6A2)
complex neurodevelopmental disorder (SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
Kleefstra syndrome (EHMT1)
mitochondrial disease (NDUFS6)
monogenic diabetes (ABCC8, PAX4)
mucopolysaccharidosis type 4A (GALNS)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (MYO7A)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 5 (HYDIN)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
Usher syndrome type 1 (MYO7A)
Other WGS-derived genes
AGL, ALDH3A2, ATXN3, AURKC, BMP4, CARD8, CASP12, CASP8, CATSPER2, CHST15, CLDN16, COLQ, CYP21A2, D2HGDH, DEFB126, DNAH9, DSC3, ERCC6L2, FCGR2A, FUT2, GALNT3, GLYCTK, GPR161, H6PD, HLA-DRB5, HSD17B13, IRF5, ITGB2, KCNJ16, LAMA5, MAPT, MROH8, MTTP, NDUFB9, NFU1, NOTCH2, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, POLR3B, PRKRA, RAD52, RNASEH2B, RXFP2, SCAPER, SH3BP2, SIGLEC12, SLC37A4, SPATA7, SRA1, TBP, TDG, TGIF1, TMEM107, TMEM216, TNRC18, TREH, TRPM1, TUBB8, VDR, VRK1, WDR37, ZAN, ZNF124