WTSIi135-A

HPSI0214i-feec_2

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi135-A
Alternative name(s)
HPSI0214i-feec_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi135-B
(HPSI0214i-feec_3)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi135-A
BioSamples SAMEA2612471
HipSci HPSI0214i-feec_2
Cellosaurus CVCL_AE51
ECACC 77650191, 66540240
CLO CLO_0101142
Wikidata Q54890535

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398079
HipSci HPSI-feec

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 60-64
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
28.573 1.418 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Exome sequencing
cnv
http://www.hipsci.org/lines/#/lines/HPSI0214i-feec_2
Number of regions different from primary tissue: 2; Length of differences from primary tissue: 4
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629686
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1821158
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127646
Genotyping array calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861325
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ447884
GATK haplotype calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR1629676
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1805410
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1821156
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376184
Splice-aware STAR alignment
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127411
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ376043
Abundances of transcripts
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, LAMC3)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
factor XIII, b subunit, deficiency of (F13B)
familial thoracic aortic aneurysm and aortic dissection (MFAP5)
hereditary nonpolyposis colon cancer (XRCC4)
hyperprolinemia type 1 (PRODH)
Leigh syndrome (HIBCH)
Lynch syndrome (PMS1)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
myopathy caused by variation in POMT1 (POMT1)
nephronophthisis 4 (NPHP4)
PHARC syndrome (ABHD12)
primary ciliary dyskinesia 5 (HYDIN)
primary ciliary dyskinesia 7 (DNAH11)
Rothmund-Thomson syndrome (RECQL4)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
vitamin K-dependent clotting factors, combined deficiency of, type 2 (VKORC1)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
ACTR3C, AHNAK, ALDH3A2, AMPD2, ANAPC1, CARD8, CASP12, CATSPER2, CHST15, CLDN16, CNOT1, COLQ, CYP21A2, DEFB126, DNAAF1, DSC3, FANCM, FBXO7, FLG2, FUT2, GALNT3, GLYCTK, GPRIN1, HLA-DRB5, HSD17B13, IDO2, IRF5, ITGB2, KCNJ16, LAMA5, MAPT, MOCOS, MROH8, MTTP, NBPF1, NDUFB9, NFU1, NPRL3, OAS1, P2RX5, P2RX7, PDE4DIP, PIGN, POLDIP2, PRKRA, PTCHD3, PYGL, RNF212, RXFP2, SIGLEC12, SLC37A4, SPATA7, SRA1, SYNE2, TGIF1, TIGD6, TMEM216, TMPRSS6, TNRC18, TOR1AIP1, TREH, TRPM1, UNC93A, UTRN, VDR, WDR37, ZAN