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WTSIi129-A
HPSI0613i-zisa_3
iPSC line
The cell line was withdrawn.
General#
Cell Line |
|
| hPSCreg name | WTSIi129-A |
| Alternative name(s) |
HPSI0613i-zisa_3
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi129-A |
| BioSamples | SAMEA2398851 |
| HipSci | HPSI0613i-zisa_3 |
| Cellosaurus | CVCL_AG38 |
| Wikidata | Q54891110 |
General Information |
|
| Publications | View all related publications on hPSCreg (2) |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor |
|
External Databases (Donor) |
|
| BioSamples | SAMEA2399101 |
| HipSci | HPSI-zisa |
hIPSC Derivation#
General |
|
| Source cell type |
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
|
| Source cell origin |
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Gelatin |
| Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
| Passage method |
Enzymatically
Collagenase and Dispase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
| Pluripotency Score | Novelty Score | Link to microarray data |
| 61.16 | 1.365 | https://www.ebi.ac.uk/ega/datasets/EGAD00010000775 |
Report
HPSI-zisa.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-zisa.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592
cnv
http://www.hipsci.org/lines/#/lines/HPSI0613i-zisa_3 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139 Text file with probe intensities |