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iPSC line

No longer available
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.


Cell Line

hPSCreg name WTSIi116-A
Alternative name(s)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.


Depositor Wellcome Sanger Institute (WTSI)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi116-A
BioSamples SAMEA2398866
HipSci HPSI0813i-iicq_1
Cellosaurus CVCL_AH08
Wikidata Q54891380

General Information

Publications View all related publications on hPSCreg (2)
This EBiSC line can be used for:
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 30-34
Ethnicity White - Other

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2397863
HipSci HPSI-iicq

hIPSC Derivation#


Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 30-34
Collected in 2013
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming


Selection criteria for clones Morphology
Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Pluripotency Score Novelty Score Link to microarray data
36.897 1.351


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
Array signal intensity data
Genotyping array
Imputed and phased genotypes
Expression array
Array signal intensity data
Abundances of transcripts
Exome sequencing
Raw sequencing reads
Splice-aware STAR alignment
Exome sequencing
Imputed and phased genotypes
Genotyping array
Array signal intensity data
Methylation profiling
Text file with probe intensities
Genotyping array
Genotyping array calls
Exome sequencing
BWA alignment
Expression array
GenomeStudio text file
Exome sequencing
mpileup variant calls