WTSIi115-A
HPSI0513i-iasn_3
iPSC line
The cell line was withdrawn.
General#
Cell Line |
|
| hPSCreg Name | WTSIi115-A |
| Alternative name(s) |
HPSI0513i-iasn_3
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi020-A (HPSI0114i-eipl_1) WTSIi021-A (HPSI1013i-wuye_2) WTSIi024-A (HPSI0114i-vabj_3) WTSIi026-A (HPSI0314i-hoik_1) WTSIi027-A (HPSI0913i-oapg_5) WTSIi028-A (HPSI1213i-babk_2) WTSIi029-A (HPSI1013i-garx_2) WTSIi030-A (HPSI0214i-pelm_3) WTSIi040-A (HPSI0314i-qaqx_1) WTSIi041-A (HPSI0114i-bezi_1) WTSIi015-B (HPSI0114i-rozh_4) WTSIi021-B (HPSI1013i-wuye_3) WTSIi044-A (HPSI0214i-eiwy_1) WTSIi045-A (HPSI0214i-heth_1) WTSIi005-B (HPSI1113i-podx_2) WTSIi046-A (HPSI0214i-wibj_2) WTSIi047-A (HPSI0814i-bokz_5) WTSIi038-B (HPSI1113i-ieki_2) WTSIi048-A (HPSI0214i-datg_2) WTSIi047-B (HPSI0814i-bokz_6) WTSIi049-A (HPSI0514i-wiii_2) WTSIi030-B (HPSI0214i-pelm_1) WTSIi049-B (HPSI0514i-wiii_3) WTSIi052-A (HPSI1114i-ziyn_6) WTSIi055-A (HPSI0513i-debk_9) WTSIi056-A (HPSI0513i-dipe_1) WTSIi057-A (HPSI0513i-giuf_3) WTSIi058-A (HPSI0513i-suzg_3) WTSIi060-A (HPSI0513i-veqz_6) WTSIi061-A (HPSI0513i-veve_2) WTSIi062-A (HPSI0513i-zaui_3) WTSIi066-A (HPSI0613i-febc_1) WTSIi067-A (HPSI0713i-foop_1) WTSIi067-B (HPSI0713i-foop_2) WTSIi071-A (HPSI0913i-lise_1) WTSIi066-B (HPSI0613i-febc_2) WTSIi012-B (HPSI0314i-bubh_3) WTSIi074-A (HPSI0114i-joxm_1) WTSIi017-B (HPSI0114i-lexy_1) WTSIi075-A (HPSI0114i-vass_1) WTSIi076-A (HPSI0214i-kehc_2) WTSIi077-A (HPSI0314i-cuhk_1) WTSIi011-B (HPSI0314i-qonc_1) WTSIi003-A (HPSI1213i-hehd_2) WTSIi005-A (HPSI1113i-podx_1) WTSIi007-A (HPSI1113i-dons_1) WTSIi009-A (HPSI0913i-diku_1) WTSIi011-A (HPSI0314i-qonc_2) WTSIi012-A (HPSI0314i-bubh_1) WTSIi015-A (HPSI0114i-rozh_5) |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi115-A |
| BioSamples | SAMEA2397877 |
| HipSci | HPSI0513i-iasn_3 |
| Cellosaurus | CVCL_AF54 |
| ECACC | 77650025 |
| CLO | CLO_0101110 |
| Wikidata | Q54890829 |
General Information |
|
| Publications | View all related publications on hPSCreg (2) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA2398924 |
| HipSci | HPSI-iasn |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Gelatin |
| Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
| Passage method |
Enzymatically
Collagenase and Dispase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
| Pluripotency Score | Novelty Score | Link to microarray data |
| 38.616 | 1.252 | https://www.ebi.ac.uk/ega/datasets/EGAD00010000775 |
Report
HPSI-iasn.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-iasn.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592
cnv
http://www.hipsci.org/lines/#/lines/HPSI0513i-iasn_3 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139 Text file with probe intensities |