WTSIi115-A
HPSI0513i-iasn_3
iPSC line
The cell line was withdrawn.
General#
Cell Line |
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hPSCreg Name | WTSIi115-A |
Alternative name(s) |
HPSI0513i-iasn_3
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
WTSIi020-A (HPSI0114i-eipl_1) WTSIi021-A (HPSI1013i-wuye_2) WTSIi024-A (HPSI0114i-vabj_3) WTSIi026-A (HPSI0314i-hoik_1) WTSIi027-A (HPSI0913i-oapg_5) WTSIi028-A (HPSI1213i-babk_2) WTSIi029-A (HPSI1013i-garx_2) WTSIi030-A (HPSI0214i-pelm_3) WTSIi040-A (HPSI0314i-qaqx_1) WTSIi041-A (HPSI0114i-bezi_1) WTSIi015-B (HPSI0114i-rozh_4) WTSIi021-B (HPSI1013i-wuye_3) WTSIi044-A (HPSI0214i-eiwy_1) WTSIi045-A (HPSI0214i-heth_1) WTSIi005-B (HPSI1113i-podx_2) WTSIi046-A (HPSI0214i-wibj_2) WTSIi047-A (HPSI0814i-bokz_5) WTSIi038-B (HPSI1113i-ieki_2) WTSIi048-A (HPSI0214i-datg_2) WTSIi047-B (HPSI0814i-bokz_6) WTSIi049-A (HPSI0514i-wiii_2) WTSIi030-B (HPSI0214i-pelm_1) WTSIi049-B (HPSI0514i-wiii_3) WTSIi052-A (HPSI1114i-ziyn_6) WTSIi055-A (HPSI0513i-debk_9) WTSIi056-A (HPSI0513i-dipe_1) WTSIi057-A (HPSI0513i-giuf_3) WTSIi058-A (HPSI0513i-suzg_3) WTSIi060-A (HPSI0513i-veqz_6) WTSIi061-A (HPSI0513i-veve_2) WTSIi062-A (HPSI0513i-zaui_3) WTSIi066-A (HPSI0613i-febc_1) WTSIi067-A (HPSI0713i-foop_1) WTSIi067-B (HPSI0713i-foop_2) WTSIi071-A (HPSI0913i-lise_1) WTSIi066-B (HPSI0613i-febc_2) WTSIi012-B (HPSI0314i-bubh_3) WTSIi074-A (HPSI0114i-joxm_1) WTSIi017-B (HPSI0114i-lexy_1) WTSIi075-A (HPSI0114i-vass_1) WTSIi076-A (HPSI0214i-kehc_2) WTSIi077-A (HPSI0314i-cuhk_1) WTSIi011-B (HPSI0314i-qonc_1) WTSIi003-A (HPSI1213i-hehd_2) WTSIi005-A (HPSI1113i-podx_1) WTSIi007-A (HPSI1113i-dons_1) WTSIi009-A (HPSI0913i-diku_1) WTSIi011-A (HPSI0314i-qonc_2) WTSIi012-A (HPSI0314i-bubh_1) WTSIi015-A (HPSI0114i-rozh_5) |
Provider |
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Depositor | Wellcome Sanger Institute (WTSI) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | United Kingdom |
External Databases |
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hPSCreg | WTSIi115-A |
BioSamples | SAMEA2397877 |
HipSci | HPSI0513i-iasn_3 |
Cellosaurus | CVCL_AF54 |
ECACC | 77650025 |
CLO | CLO_0101110 |
Wikidata | Q54890829 |
General Information |
|
Publications | View all related publications on hPSCreg (2) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA2398924 |
HipSci | HPSI-iasn |
hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
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Source cell line vendor | Cambridge BioResource |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Genes | |
Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
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Other |
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Selection criteria for clones | Morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Gelatin |
Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
Passage method |
Enzymatically
Collagenase and Dispase
|
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SOX2 |
Yes |
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NANOG |
Yes |
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Pluripotency Score | Novelty Score | Link to microarray data |
38.616 | 1.252 | https://www.ebi.ac.uk/ega/datasets/EGAD00010000775 |
Report
HPSI-iasn.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-iasn.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592
cnv
http://www.hipsci.org/lines/#/lines/HPSI0513i-iasn_3 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139 Text file with probe intensities |