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WTSIi115-A

HPSI0513i-iasn_3

iPSC line

No longer available
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi115-A
Alternative name(s)
HPSI0513i-iasn_3
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi115-A
BioSamples SAMEA2397877
HipSci HPSI0513i-iasn_3
Cellosaurus CVCL_AF54
Wikidata Q54890829

General Information

Publications View all related publications on hPSCreg (2)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398924
HipSci HPSI-iasn

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
38.616 1.252 https://www.ebi.ac.uk/ega/datasets/EGAD00010000775

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0513i-iasn_3
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Expression array
https://ega-archive.org/datasets/EGAD00010001143
GenomeStudio text file
Expression array
https://ega-archive.org/datasets/EGAD00010001143
Array signal intensity data
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Imputed and phased genotypes
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Raw sequencing reads
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Text file with probe intensities
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Abundances of transcripts
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
BWA alignment