WTSIi107-A

HPSI0813i-iill_1

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name WTSIi107-A
Alternative name(s)
HPSI0813i-iill_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
WTSIi107-B
(HPSI0813i-iill_3)

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country United Kingdom

External Databases

hPSCreg WTSIi107-A
BioSamples SAMEA2398643
HipSci HPSI0813i-iill_1
Cellosaurus CVCL_AH10
ECACC 77650075, 66540222
CLO CLO_0101096
Wikidata Q54891383

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2398961
HipSci HPSI-iill

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Age of donor (at collection) 60-64
Collected in 2013
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Passage method EDTA
Surface coating Vitronectin
O2 concentration 20
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
39.923 1.323 https://www.ebi.ac.uk/ega/datasets/EGAD00010000775

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0813i-iill_1
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Expression array
https://ega-archive.org/datasets/EGAD00010001143
GenomeStudio text file
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Imputed and phased genotypes
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Array signal intensity data
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
Raw sequencing reads
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Genotyping array calls
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Abundances of transcripts
RNA-seq
https://ega-archive.org/datasets/EGAD00001003529
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
BWA alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003514
mpileup variant calls
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Array signal intensity data
Expression array
https://ega-archive.org/datasets/EGAD00010001143
Array signal intensity data
Genotyping array
https://ega-archive.org/datasets/EGAD00010001147
Imputed and phased genotypes
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139
Text file with probe intensities