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WTSIi103-A
HPSI1213i-nusw_2
iPSC line
The cell line was withdrawn.
General#
Cell Line |
|
| hPSCreg name | WTSIi103-A |
| Alternative name(s) |
HPSI1213i-nusw_2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi103-A |
| BioSamples | SAMEA2464779 |
| HipSci | HPSI1213i-nusw_2 |
| Cellosaurus | CVCL_AI20 |
| Wikidata | Q54891724 |
General Information |
|
| Publications | View all related publications on hPSCreg (2) |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 65-69 |
| Ethnicity | White - White British |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA2398815 |
| HipSci | HPSI-nusw |
hIPSC Derivation#
General |
|
| Source cell type |
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
|
| Source cell origin |
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
| Age of donor (at collection) | 65-69 |
| Collected in | 2014 |
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Gelatin |
| Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
| Passage method |
Enzymatically
Collagenase and Dispase
|
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
||||
| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
| Pluripotency Score | Novelty Score | Link to microarray data |
| 35.818 | 1.861 | http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/ |
Report
HPSI-nusw.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-nusw.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122995
cnv
http://www.hipsci.org/lines/#/lines/HPSI1213i-nusw_2 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 4
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/ Text file with probe intensities |
| WGS-derived disease associations |
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Bernard-Soulier syndrome (GP1BA)
Brugada syndrome 1 (CACNB2)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
familial ovarian cancer (GEN1)
Fanconi anemia complementation group A (FANCA)
hereditary breast carcinoma (GEN1)
hereditary nonpolyposis colon cancer (XRCC4)
hypertrophic cardiomyopathy (CACNB2)
immunodeficiency, common variable, 3 (CD19)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
platelet-type von Willebrand disease (GP1BA)
PTEN hamartoma tumor syndrome (PTEN)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
short QT syndrome (CACNB2)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
|
| Other WGS-derived genes | AGAP1, AGL, ALDH3A2, AMPD1, ANAPC1, ATP8B3, AURKC, C6, CASP12, CEP170, CHIT1, CHST15, CNOT1, COLQ, CYP21A2, D2HGDH, DSC3, ERCC6L2, FBXO7, FLG2, FUT2, GALNT3, GDPD4, HSD17B13, ITGB2, KCNJ16, LAMA5, LPL, MAPT, MESP1, MROH8, NDUFB9, NOTCH2, NPRL3, OAS1, OR1B1, OR51F1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, RNF212, SCAPER, SELPLG, SIGLEC12, SLC37A4, SPTBN5, SYNE2, TAP2, TBP, TIGD6, TLR5, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, VRK1, WDR37, ZAN, ZNF141, ZNF83, ZNF844 |