WTSIi093-A
HPSI0413i-sukz_1
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | WTSIi093-A |
| Alternative name(s) |
HPSI0413i-sukz_1
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WTSIi002-A (HPSI0913i-eika_2) WTSIi004-A (HPSI1113i-qolg_3) WTSIi006-A (HPSI1113i-hayt_1) WTSIi008-A (HPSI1013i-kuxp_1) WTSIi010-A (HPSI0314i-xugn_1) WTSIi013-A (HPSI0214i-kucg_2) WTSIi014-A (HPSI0214i-heja_2) WTSIi016-A (HPSI0114i-oevr_3) WTSIi018-A (HPSI0114i-kolf_3) WTSIi019-A (HPSI0114i-iisa_3) WTSIi022-A (HPSI1013i-yemz_1) WTSIi023-A (HPSI0114i-zapk_3) WTSIi025-A (HPSI1013i-pamv_1) WTSIi031-A (HPSI0314i-bipt_1) WTSIi032-A (HPSI1113i-bima_1) WTSIi039-A (HPSI1013i-wopl_1) WTSIi042-A (HPSI1013i-jufd_2) WTSIi014-B (HPSI0214i-heja_1) WTSIi010-B (HPSI0314i-xugn_2) WTSIi025-B (HPSI1013i-pamv_3) WTSIi004-B (HPSI1113i-qolg_1) WTSIi022-B (HPSI1013i-yemz_3) WTSIi006-B (HPSI1113i-hayt_3) WTSIi019-B (HPSI0114i-iisa_1) WTSIi050-A (HPSI1014i-toss_3) WTSIi051-A (HPSI1113i-uofv_1) WTSIi053-A (HPSI0413i-corn_1) WTSIi054-A (HPSI0413i-nudd_1) WTSIi064-A (HPSI0613i-bohf_1) WTSIi065-A (HPSI0613i-eojr_2) WTSIi068-A (HPSI0613i-giuo_5) WTSIi070-A (HPSI0713i-terl_1) WTSIi072-A (HPSI0114i-fikt_3) WTSIi065-B (HPSI0613i-eojr_3) WTSIi070-B (HPSI0713i-terl_2) WTSIi042-B (HPSI1013i-jufd_3) WTSIi078-A (HPSI0314i-fafq_1) WTSIi079-A (HPSI1013i-hiaf_2) WTSIi081-A (HPSI1113i-qorq_2) WTSIi084-A (HPSI0713i-cicb_3) WTSIi090-A (HPSI0813i-robp_3) WTSIi091-A (HPSI0813i-ffdc_11) WTSIi092-A (HPSI0713i-nemb_1) WTSIi095-A (HPSI0813i-vajx_2) WTSIi101-A (HPSI0513i-fejf_2) WTSIi106-A (HPSI0613i-hikj_2) WTSIi107-A (HPSI0813i-iill_1) WTSIi081-B (HPSI1113i-qorq_1) WTSIi084-B (HPSI0713i-cicb_2) WTSIi107-B (HPSI0813i-iill_3) |
Provider |
|
| Depositor | Wellcome Sanger Institute (WTSI) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | United Kingdom |
External Databases |
|
| hPSCreg | WTSIi093-A |
| BioSamples | SAMEA2398007 |
| HipSci | HPSI0413i-sukz_1 |
| Cellosaurus | CVCL_AF03 |
| ECACC | 77650118, 66540208 |
| CLO | CLO_0101075 |
| Wikidata | Q54890677 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA2399253 |
| HipSci | HPSI-sukz |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
| Source cell line vendor | Cambridge BioResource |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Genes | |
| Notes on reprogramming vector detection | CytoTune 1 |
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | Morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Passage method | EDTA |
| Surface coating | Vitronectin |
| O2 concentration | 20 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|
|
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| SOX2 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
| Pluripotency Score | Novelty Score | Link to microarray data |
| 29.192 | 1.225 | https://www.ebi.ac.uk/ega/datasets/EGAD00010000775 |
Report
HPSI-sukz.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-sukz.pluritest.novelty_score.20161010.png
novelty image
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
https://www.ebi.ac.uk/ega/studies/EGAS00001000592
cnv
http://www.hipsci.org/lines/#/lines/HPSI0413i-sukz_1 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 4
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001139 Text file with probe intensities |